Phylogeny and Active Ingredients of Artificial Ophiocordyceps Lanpingensis Ascomata

To evaluate the morphological character, phylogenesis and functional components of artificial Ophiocordyceps lanpingensis, a related species of O. sinensis. The ascomata of O. lanpingensis was induced with its asexual strain, HLANY0707 and its microscopic feature was described. Phylogenesis was analyzed with ITS-5.8S sequences of HLANY0707, its cultured stroma, and 39 relative sequences of Hirsutella and Ophiocordyceps based on the maximum likelihood tree. Six nucleosides of artificial O. lanpingensis, natural O. lanpingensis and natural O. sinensis were compared with HPLC analysis. Artificial ascomata of O. lanpingensis could be massively produced with HLANY0707 and had similar microscopic features as the nature specimens. Phylogenetic analysis showed that both the artificial and natural O. lanpingensis had closer relationship with O. sinensis, O. xuefengensis, H. uncinata and O. robertsii, the species whose massively cultured ascomata being not reported. Nucleosides of artificial O. lanpingensis were very similar to natural O. sinensis, implying a promising application prospect of artificial O. lanpingensis as an alternative to O. sinensis. It showed a promising way to develop artificial O. lanpingensis and conserve the rare and endangered species, O. sinensis.