Overexpression of E2F-1 inhibits progression of gastric cancer in vitro

被引：26

作者：

Xie, Yubo ^{[1
,2
]}

Wang, Changqing ^{[1
]}

Li, Lei ^{[1
]}

Ma, Yulin ^{[1
]}

Yin, Yongshuo ^{[1
]}

Xiao, Qiang ^{[1
]}

机构：

[1] Guangxi Med Univ, Affiliated Hosp 1, Dept Surg, Nanning 530021, Guangxi, Peoples R China

[2] Guangxi Med Univ, Affiliated Hosp 1, Dept Anesthesiol, Nanning 530021, Guangxi, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 06期

基金：

芬兰科学院;

关键词：

E2F-1; Overexpression; Gastric cancer; MGC-803; cells; Tumor progression; PROTEIN-KINASE PKR; NF-KAPPA-B; GENE-TRANSFER; E2F-1-INDUCED APOPTOSIS; TRIGGERS APOPTOSIS; CELL-LINES; C-MYC; TRANSCRIPTION; EXPRESSION; GROWTH;

D O I：

10.1016/j.cellbi.2009.02.015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

E2F-1 plays a critical role in cell cycle regulation and other biological processes in cells. E2F-1 mediates apoptosis and suppresses tumorigenesis in many tissue types, but there are few data available on E2F-1 expression and its relationship to tumor kinetics in gastric cancer. To gain better insight into the involvement of E2F-1 in the biological characteristics of gastric tumors, we investigated the effect of E2F-1 overexpression on the progression of gastric carcinoma cells. A gastric cancer cell line stably overexpressing E2F-1 (MGC-803/E2F-1) was established. The influence of E2F-1 overexpression on in vitro cell growth was assessed by measuring cell survival, colony formation, and cell cycle progression. The results clearly show that overexpression of E2F-1 significantly inhibits cell growth and proliferation, blocking entry into the S-phase of the cell cycle. MGC-803/E2F-1 cells also had a higher apoptotic rate than control cells. In addition, E2F-1 reduced the motility and invasion of gastric cancer cells. (c) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：640 / 649

页数：10

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