A Near-Infrared Fluorescent Nanosensor (AuC@Urease) for the Selective Detection of Blood Urea

被引：65
作者：
Nair, Lakshmi V. [1 ]
Philips, Divya S. [2 ]
Jayasree, Ramapurath S. [1 ]
Ajayaghosh, Ayyappanpillai [2 ]
机构：
[1] Sree Chitra Tirunal Inst Med Sci & Technol, Biophoton & Imaging Lab, Trivandrum 695012, Kerala, India
[2] CSIR, NIIST, Chem Sci & Technol Div, Photosci & Photon Grp, Trivandrum 695019, Kerala, India
来源：
SMALL | 2013年 / 9卷 / 16期
关键词：
gold quantum clusters; nanobiosensors; urease; urea sensors; whole blood analysis; GOLD NANOPARTICLES; METAL NANOPARTICLES; OPTICAL-PROPERTIES; VISUAL DETECTION; CLUSTERS; SENSORS; IMMOBILIZATION; BIOSENSOR; ENZYMES; MILK;
D O I：
10.1002/smll.201300213
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The use of a nanosensor (AuC@Urease) based on an NIR-emitting gold cluster and urease enzyme selectively detects urea in whole blood. The detection is based on an enzyme-specific conversion of urea to ammonium ions which facilitates pH-induced aggregation of AuC, leading to fluorescence quenching. This method does not interfere with urease inactive analytes or the autofluorescence of blood as confirmed by comparable urea levels obtained by AuC@Urease and standard clinical methods within an error limit of ±3%. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：2673 / 2677
页数：5
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