5-HTP decreases goat mammary epithelial cells apoptosis through MAPK/ERK/Bcl-3 pathway

被引：14

作者：

Zhao, Haiying ^{[1
]}

Chen, Shunxin ^{[1
]}

Hu, Kaizhao ^{[1
]}

Zhang, Zhifei ^{[1
]}

Yan, Xiaoru ^{[1
]}

Gao, Huijie ^{[1
]}

Du, Wei ^{[1
]}

Zheng, Huiling ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling 712100, Shaanxi, Peoples R China

来源：

GENE | 2021年 / 769卷

基金：

中国国家自然科学基金;

关键词：

5-HT; Apoptosis; Goat; ERK1/2; Bcl-3; NF-KAPPA-B; ANAPLASTIC LARGE-CELL; FAMILY-MEMBER BCL-3; T-CELL; BREAST-CANCER; SEROTONIN; PROLIFERATION; ACTIVATION; GLAND; EXPRESSION;

D O I：

10.1016/j.gene.2020.145240

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Serotonin (5-HT) is a monoamine and it could regulate cell growth by its receptors working on signaling pathways. 5-HTP is the precursor of 5-HT that help 5-HT synthesis. B cell leukemia/lymphoma 3 (Bcl-3) involved in cell death and proliferation through mitogen activated protein kinase (MAPK) pathway. However, there is little information about the effects of MAPK/Bcl-3 on apoptosis of goat mammary gland epithelial cells (GMECs). The aim of this study is to explore the interaction among 5-HTP, MAPK and Bcl-3 in GMEC apoptosis. In this study, 5-HTP treatment decreased cell apoptosis and promoted phosphorylation of ERK1/2 in GMEC. We also found that the activation and inhibition of ERK1/2 could affect GMEC apoptosis. The Annexin V-FITC/PI staining and western blotting results suggested that 5-HTP decreased GMEC apoptosis through ERK1/2 signaling pathway. And the results of RT-qPCR and western blotting demonstrated that both 5-HTP and ERK1/2 positively regulated Bcl-3 expression. Sum up all the results, we could draw the conclusion that 5-HTP decreased GMEC apoptosis through MAPK/ERK/Bcl-3 pathway.

引用

页数：7

共 44 条

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