Lipopolysaccharide-induced expression of inducible nitric oxide synthase in the guinea pig organ of Corti

被引:44
作者
Takumida, M
Anniko, M
Ropa, P
Zhang, DM
机构
[1] Hiroshima Univ, Sch Med, Dept Otolaryngol, Minami Ku, Hiroshima 7348551, Japan
[2] Univ Uppsala Hosp, Dept Otolaryngol Head & Neck Surg, Uppsala, Sweden
关键词
nitric oxide; inducible nitric oxide synthase; bacterial lipopolysaccharide; organ of Corti; immunohistochemistry; guinea pig;
D O I
10.1016/S0378-5955(99)00188-4
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
The purpose of the investigation was to ascertain whether inoculation of bacterial lipopolysaccharide (LP) into the cochlea of the guinea pig could elicit formation of inducible nitric oxide synthase (iNOS). Immunohistochemical study revealed that immunoreactivity to iNOS was seen below outer hair cells representing nerve fibers and synaptic nerve endings. iNOS-staining could also be observed ill phalangeal dendrites of Deiter's cells pointing to the cuticular membrane, Hensen's cells and on stria vascularis 48 h after inoculation with LPS. Immunohistochemical investigation with a specific anti-nitrotyrosine antibody also revealed intense immunoreactivity identical to that of iNOS, suggesting formation of peroxynitrite in the organ of Corti by the reaction of NO with O-2(-). On the basis of these findings, it can be concluded that NO together with O-2(-), which form the more reactive peroxynitrite. are the most important pathogenic agents in LPS-induced damage of cochlea in the guinea pig. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:91 / 98
页数:8
相关论文
共 38 条
[1]   N-G-METHYL-L-ARGININE PROTECTS THE GUINEA-PIG COCHLEA FROM THE CYTOTOXIC EFFECTS OF PNEUMOLYSIN [J].
AMAEE, FR ;
COMIS, SD ;
OSBORNE, MP .
ACTA OTO-LARYNGOLOGICA, 1995, 115 (03) :386-391
[2]   Possible involvement of nitric oxide in the sensorineural hearing loss of bacterial meningitis [J].
Amaee, FR ;
Comis, SD ;
Osborne, MP ;
Drew, S ;
Tarlow, MJ .
ACTA OTO-LARYNGOLOGICA, 1997, 117 (03) :329-336
[3]  
Beckman JS, 1996, AM J PHYSIOL-CELL PH, V271, pC1424
[4]  
CHAO CC, 1992, J IMMUNOL, V149, P2736
[5]  
Dais CGD, 1996, HEARING RES, V99, P1
[6]   GLUTAMATE RECEPTORS IN AFFERENT COCHLEAR NEUROTRANSMISSION IN GUINEA-PIGS [J].
EHRENBERGER, K ;
FELIX, D .
HEARING RESEARCH, 1991, 52 (01) :73-80
[7]   The nitric oxide/cyclic GMP pathway: A potential major regulator of cochlear physiology [J].
Fessenden, JD ;
Schacht, J .
HEARING RESEARCH, 1998, 118 (1-2) :168-176
[8]   Localization of soluble guanylate cyclase activity in the guinea pig cochlea suggests involvement in regulation of blood flow and supporting cell physiology [J].
Fessenden, JD ;
Schacht, J .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1997, 45 (10) :1401-1408
[9]   DETECTION AND CHARACTERIZATION OF NITRIC-OXIDE SYNTHASE IN THE MAMMALIAN COCHLEA [J].
FESSENDEN, JD ;
COLING, DE ;
SCHACHT, J .
BRAIN RESEARCH, 1994, 668 (1-2) :9-15
[10]   NITRIC-OXIDE SYNTHASE ISOZYMES - CHARACTERIZATION, PURIFICATION, MOLECULAR-CLONING, AND FUNCTIONS [J].
FORSTERMANN, U ;
CLOSS, EI ;
POLLOCK, JS ;
NAKANE, M ;
SCHWARZ, P ;
GATH, I ;
KLEINERT, H .
HYPERTENSION, 1994, 23 (06) :1121-1131