Development and characterization of highly polymorphic microsatellite loci for the Western Spadefoot toad, Pelobates cultripes

被引：4

作者：

van de Vliet, Mirjam S. ^{[1
]}

Diekmann, Onno E. ^{[1
]}

Serrao, Ester T. A. ^{[1
]}

Beja, Pedro ^{[2
,3
]}

机构：

[1] Univ Algarve, CCMAR CIMAR, FCMA, P-8005139 Faro, Portugal

[2] ERENA Ordenamento & Gestao, Recursos Nat Lda, P-1900392 Lisbon, Portugal

[3] CIBIO & Recursos Genet, P-448566 Vairao, Portugal

来源：

CONSERVATION GENETICS | 2009年 / 10卷 / 04期

关键词：

Pelobates cultripes; Western Spadefoot; Pelobates fuscus; Microsatellites; Cross-species amplification; Temporary ponds; Amphibian conservation genetics; INTENSIFICATION;

D O I：

10.1007/s10592-008-9670-6

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Nine highly polymorphic microsatellite markers were isolated and characterized for the Western Spadefoot, Pelobates cultripes. Remarkably, for this amphibian species high numbers of microsatellites were found as part of larger repeat containing regions, making primer design difficult. For nine loci, primers were designed successfully and genotyping of individuals was reliable and consistent. Number of alleles and heterozygosity for these loci ranged from 9 to 34 and from 0.72 to 0.94, respectively. The high levels of polymorphism revealed by our developed loci should provide insight into population genetic structure and levels of dispersal for this typical Mediterranean temporary pond-breeding amphibian.

引用

页码：993 / 996

页数：4

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