Radiobiological response of U251MG, CHO-K1 and V79 cell lines to accelerator-based boron neutron capture therapy

被引：30

作者：

Sato, Eisuke ^{[1
,2
]}

Zaboronok, Alexander ^{[3
]}

Yamamoto, Tetsuya ^{[4
]}

Nakai, Kei ^{[5
]}

Taskaev, Sergey ^{[6
,7
]}

Volkova, Olga ^{[7
,8
]}

Mechetina, Ludmila ^{[7
,8
]}

Taranin, Alexander ^{[7
,8
]}

Kanygin, Vladimir ^{[6
,7
,9
]}

Isobe, Tomonori ^{[3
]}

Mathis, Bryan J. ^{[3
]}

Matsumura, Akira ^{[3
]}

机构：

[1] Univ Tsukuba, Grad Sch Comprehens Human Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan

[2] Kyorin Univ, Fac Hlth Sci, 5-4-1 Shimorenjaku, Mitaka, Tokyo 1818612, Japan

[3] Univ Tsukuba, Fac Med, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan

[4] Yokohama City Univ, Grad Sch Med, Dept Neurosurg, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan

[5] Ibaraki Prefectural Univ Hlth Sci, 4669-2 Ami, Inashiki, Ibaraki 3000331, Japan

[6] Budker Inst Nucl Phys, Lavrentieva Prosp 11, Novosibirsk 630090, Russia

[7] Novosibirsk State Univ, Pirogova Str 2, Novosibirsk 630090, Russia

[8] Inst Mol & Cell Biol, Lavrentieva Prosp 8-2, Novosibirsk 630090, Russia

[9] Novosibirsk State Med Univ, Krasny Prosp 52, Novosibirsk 630091, Russia

来源：

JOURNAL OF RADIATION RESEARCH | 2018年 / 59卷 / 02期

基金：

俄罗斯科学基金会;

关键词：

boron neutron capture therapy; accelerator-based neutron source; lithium target; boric acid; in vitro efficacy evaluation; RECURRENT HEAD; NECK-CANCER; IN-VITRO; TARGET; BNCT; GLIOBLASTOMA; NANOPARTICLES; TRANSPORT; TSUKUBA;

D O I：

10.1093/jrr/rrx071

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In the current article, we provide in vitro efficacy evaluation of a unique accelerator-based neutron source, constructed at the Budker Institute of Nuclear Physics (Novosibirsk, Russian Federation), for boron neutron capture therapy (BNCT), which is particularly effective in the case of invasive cancers. U251MG, CHO-K1 and V79 cells were incubated and irradiated in various concentrations of boric acid with epithermal neutrons for 2-3 h in a plexiglass phantom, using 2.0 MeV proton energy and 1.5-3.0 mA proton current, resulting in a neutron fluence of 2.16 x 10(12) cm(-2). The survival curves of cells loaded with boron were normalized to those irradiated without boron (to exclude the influence of the fast neutron and gamma dose components) and fit to the linear-quadratic (LQ) model. Colony formation assays showed the following cell survival rates (means +/- SDs): CHO-K1: 0.348 +/- 0.069 (10 ppm), 0.058 +/- 0.017 (20 ppm), 0.018 +/- 0.005 (40 ppm); V79: 0.476 +/- 0.160 (10 ppm), 0.346 +/- 0.053 (20 ppm), 0.078 +/- 0.015 (40 ppm); and U251MG: 0.311 +/- 0.061 (10 ppm), 0.131 +/- 0.022 (20 ppm), 0.020 +/- 0.010 (40 ppm). The difference between treated cells and controls was significant in all cases (P < 0.01) and confirmed that the neutron source and irradiation regimen were sufficient for control over cell colony formation. We believe our study will serve as a model for ongoing in vitro experiments on neutron capture therapy to advance in this area for further development of accelerator-based BNCT into the clinical phase.

引用

页码：101 / 107

页数：7

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