Determination of Tetracyclines in Milk by Graphene-Based Solid-Phase Extraction and High-Performance Liquid Chromatography

被引：34

作者：

Wu, Xia Jun ^{[1
]}

Wang, Geng Nan ^{[1
]}

Yang, Kun ^{[1
]}

Liu, Hui Zhi ^{[1
]}

Wang, Jian Ping ^{[1
]}

机构：

[1] Agr Univ Hebei, Coll Vet Med, Lekai South St 2596, Baoding 071000, Hebei, Peoples R China

来源：

ANALYTICAL LETTERS | 2017年 / 50卷 / 04期

关键词：

Graphene; high-performance liquid chromatography; milk; solid-phase extraction; tetracyclines; TANDEM MASS-SPECTROMETRY; ENVIRONMENTAL WATER SAMPLES; MULTI-RESIDUE ANALYSIS; BOVINE-MILK; CARBAMATE PESTICIDES; SCREENING METHOD; ANTIBIOTICS; VALIDATION; OXYTETRACYCLINE; NANOCOMPOSITE;

D O I：

10.1080/00032719.2016.1194853

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A graphene-based solid-phase extraction (SPE) column was prepared for the isolation of tetracyclines from milk followed by determination by high-performance liquid chromatography. Graphene provided better separation for tetracyclines than amine-modified graphene and carboxyl-modified graphene. The optimized graphene-based SPE column showed high absorption capacities (greater than 4,660 ng) and high recoveries (exceeding 92%) for tetracycline, oxytetracycline, chlortetracycline, and doxycycline and was successfully reused at least fifty times. The limits of detection in milk were from 10 to 20 ng/mL, with recoveries between 82.3 and 103.6%. Furthermore, the system showed superior performance than two commercial SPE cartridges with respect to recovery, purification, and reusability. Therefore, this approach is suitable for the determination of tetracyclines in milk.

引用

页码：641 / 650

页数：10

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