Hidden abnormalities and novel classification of t(15;17) acute promyelocytic leukemia (APL) based on genomic alterations

被引:46
作者
Akagi, Tadayuki [1 ]
Shih, Lee-Yung [2 ,3 ]
Kato, Motohiro [4 ]
Kawamata, Norihiko [1 ]
Yamamoto, Go [4 ,5 ]
Sanada, Masashi [4 ,5 ]
Okamoto, Ryoko [1 ]
Miller, Carl W. [1 ]
Liang, Der-Cherng [6 ]
Ogawa, Seishi [4 ]
Koeffler, H. Phillip [1 ]
机构
[1] Univ Calif Los Angeles, Cedars Sinai Med Ctr, Sch Med, Div Hematol & Oncol, Los Angeles, CA 90048 USA
[2] Chang Gung Mem Hosp, Dept Internal Med, Div Hematol Oncol, Taipei 10591, Taiwan
[3] Chang Gung Univ, Sch Med, Tao Yuan, Taiwan
[4] Univ Tokyo, Dept Hematol & Oncol, Tokyo, Japan
[5] Univ Tokyo, Grad Sch Med, 21st Century COE Program, Tokyo, Japan
[6] Mackay Mem Hosp, Div Pediat Hematol Oncol, Taipei, Taiwan
基金
美国国家卫生研究院;
关键词
ACUTE MYELOID-LEUKEMIA; PML-RAR-ALPHA; INTERNAL TANDEM DUPLICATION; ACQUIRED UNIPARENTAL DISOMY; RETINOIC ACID; NORMAL KARYOTYPE; CELL-LINE; T(15-17) TRANSLOCATION; SIGNAL-TRANSDUCTION; PML/RAR-ALPHA;
D O I
10.1182/blood-2007-12-130260
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Acute promyelocytic leukemia (APL) is ahematopoietic malignant disease characterized by the chromosomal translocation t(15; 17), resulting in the formation of the PML-RARA gene. Here, 47 t(15; 17) APL samples were analyzed with high-density single-nucleotide polymorphism microarray (50-K and 250-K SNP-chips) using the new algorithm AsCNAR (allele-specific copy-number analysis using anonymous references). Copy-number-neutral loss of heterozygosity (CNN-LOH) was identified at chromosomes 10q(3 cases), 11p (3 cases), and 19q (1 case). Twenty-eight samples (60%) did not have an obvious alteration (normal-copy-number [NC] group). Nineteen samples (40%) showed either one or more genomic abnormalities: 8 samples (17%) had trisomy 8 either with or without an additional duplication, deletion, or CNN-LOH (+ 8 group); and 11 samples (23%) had genomic abnormalities without trisomy 8 (other abnormalities group). These chromosomal abnormalities were acquired somatic mutations. Interestingly, FLT3-ITD mutations (11/47 cases) occurred only in the group with no genomic alteration (NC group). Taken together, these results suggest that the pathway of development of APL differs in each group: FLT3-ITD, trisomy 8, and other genomic changes. Here, we showed for the first time hidden abnormalities and novel disease-related genomic changes in t(15; 17) APL. (Blood. 2009; 113: 1741-1748)
引用
收藏
页码:1741 / 1748
页数:8
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