Exogenous miRNAs from Moringa oleifera Lam. recover a dysregulated lipid metabolism

被引：9

作者：

Roglia, Valentina ^{[1
]}

Potesta, Marina ^{[1
,2
]}

Minchella, Alessandra ^{[1
]}

Bruno, Stefania Paola ^{[3
,4
]}

Bernardini, Roberta ^{[5
]}

Lettieri-Barbato, Daniele ^{[1
,6
]}

Iacovelli, Federico ^{[1
]}

Gismondi, Angelo ^{[1
]}

Aquilano, Katia ^{[1
]}

Canini, Antonella ^{[1
]}

Muleo, Rosario ^{[7
]}

Colizzi, Vittorio ^{[1
,2
]}

Mattei, Maurizio ^{[2
,5
]}

Minutolo, Antonella ^{[1
,8
]}

Montesano, Carla ^{[1
,2
]}

机构：

[1] Univ Roma Tor Vergata, Dept Biol, Rome, Italy

[2] UNESCO Interdisciplinary Chair Biotechnol & Bioet, Rome, Italy

[3] Bambino Gesu Childrens Hosp IRCCS, Rome, Italy

[4] Univ Roma Tre, Dept Sci, Rome, Italy

[5] Univ Roma Tor Vergata, Interdept Ctr Anim Technol, Rome, Italy

[6] Santa Lucia Fdn IRCCS, Rome, Italy

[7] Univ Tuscia, Dept Agr & Forestry Sci, Viterbo, Italy

[8] Univ Rome Vergata, Dept Expt Med, Rome, Italy

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2022年 / 9卷

关键词：

plant miRNAs; Moringa oleifera Lam; cross-kingdom interactions; lipid metabolism; inflammatioin; FATTY LIVER-DISEASE; ADIPOSE-TISSUE; PLANT MICRORNAS; COW MILK; INFLAMMATION; OBESITY; DIFFERENTIATION; APOPTOSIS; DIGESTION; GROWTH;

D O I：

10.3389/fmolb.2022.1012359

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A balanced diet is critical for human health, and edible plants play an important role in providing essential micronutrients as well as specific microRNAs (miRNAs) that can regulate human gene expression. Here we present the effects of Moringa oleifera (MO) miRNAs (mol-miRs) on lipid metabolism. Through in silico studies we identified the potential genes involved in lipid metabolism targeted by mol-miRs. To this end, we tested the efficacy of an aqueous extract of MO seeds (MOES), as suggested in traditional African ethnomedicine, or its purified miRNAs. The biological properties of MO preparations were investigated using a human derived hepatoma cell line (HepG2) as a model. MOES treatment decreased intracellular lipid accumulation and induced apoptosis in HepG2. In the same cell line, transfection with mol-miRs showed similar effects to MOES. Moreover, the effect of the mol-miR pool was investigated in a pre-obese mouse model, in which treatment with mol-miRs was able to prevent dysregulation of lipid metabolism.

引用

页数：15

共 88 条

[11] Commercial Dairy Cow Milk microRNAs Resist Digestion under Simulated Gastrointestinal Tract Conditions [J].