Puerarin improves intestinal barrier function through enhancing goblet cells and mucus barrier

被引：41
作者：
Li, Jiaojiao [1 ,2 ]
Zhang, Li [1 ,2 ]
Li, Yafei [3 ,4 ]
Wu, Yi [1 ,2 ]
Wu, Tao [1 ,2 ]
Feng, Hua [1 ,2 ]
Xu, Zhenjiang [1 ,2 ]
Liu, Yuhuan [1 ,2 ]
Ruan, Zheng [1 ,2 ,5 ,6 ]
Zhou, Shicheng [6 ]
机构：
[1] Nanchang Univ, Nanchang Key Lab Fruits & Vegetables Nutr & Proc, State Key Lab Food Sci & Technol, Inst Nutr, Nanchang 330047, Jiangxi, Peoples R China
[2] Nanchang Univ, Sch Food Sci, Nanchang 330047, Jiangxi, Peoples R China
[3] Nanchang Univ, Univ Campus Hosp, Nanchang 330006, Jiangxi, Peoples R China
[4] Nanchang Univ, Jiangxi Acad Med Sci, Nanchang 330006, Jiangxi, Peoples R China
[5] Beijing Technol & Business Univ BTBU, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100048, Peoples R China
[6] Jiangxi Engn Res Ctr Pueraria, Shangrao 334300, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2020年 / 75卷
关键词：
Intestinal barrier; Mucin; Goblet cells; Puerarin; Indole-3-propionic acid; GUT MICROBIOTA; EXPRESSION; MICE;
D O I：
10.1016/j.jff.2020.104246
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Puerarin is a kind of isoflavones in Puerariae Lobatae Radix with physiological activities, such as anti-inflammatory and vasodilatory activities. The aim of this study was to investigate the effects of puerarin on intestinal barrier dysfunction induced by 2,4,6 trinitrobenzene sulfonic acid (TNBS) and its underlying mechanism. Puerarin treatment attenuated the mucus barrier dysfunction, and increased the level of mucins (Muc2 and Muc4) and the number of goblet cells compared to TNBS group. Puerarin treatment significantly increased the content of indole-3-propionic acid, compared to TNBS group, and not indoleacrylic acid and butyrate. Puerarin treatment changed the structure of the commensal microbiota as evidenced by the obvious decrease in the relative abundances of Bacteroidetes. Additionally, puerarin increased the proportion of the mucin-utilizing bacterium Akkermansia. Puerarin improves intestinal barrier function through enhancing goblet cells and mucus barrier. Food enriched with puerarin could protect and repair intestinal barrier for improving gut health.
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页数：8
相关论文
共 37 条
[1]   Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota [J].
Beards, Emma ;
Tuohy, Kieran ;
Gibson, Glenn .
ANAEROBE, 2010, 16 (04) :420-425
[2]   Intestinal microbiota: A source of novel biomarkers in inflammatory bowel diseases? [J].
Berry, David ;
Reinisch, Walter .
BEST PRACTICE & RESEARCH CLINICAL GASTROENTEROLOGY, 2013, 27 (01) :47-58
[3]   Interactions of commensal and pathogenic microorganisms with the mucus layer in the colon [J].
Cai, Rui ;
Cheng, Chen ;
Chen, Jianwei ;
Xu, Xiaoqiang ;
Ding, Chao ;
Gu, Bing .
GUT MICROBES, 2020, 11 (04) :680-690
[4]   Leaky gut: mechanisms, measurement and clinical implications in humans [J].
Camilleri, Michael .
GUT, 2019, 68 (08) :1516-1526
[5]   Layered defense: how mucus and tight junctions seal the intestinal barrier [J].
Capaldo, Christopher T. ;
Powell, Domonica N. ;
Kalman, Daniel .
JOURNAL OF MOLECULAR MEDICINE-JMM, 2017, 95 (09) :927-934
[6]   Puerariae Lobatae Radix with chuanxiong Rhizoma for treatment of cerebral ischemic stroke by remodeling gut microbiota to regulate the brain-gut barriers [J].
Chen, Runzhi ;
Wu, Peng ;
Cai, Zheng ;
Fang, Yingying ;
Zhou, Hao ;
Lasanajak, Yi ;
Tang, Lan ;
Ye, Ling ;
Hou, Chuqi ;
Zhao, Jie .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2019, 65 :101-114
[7]   A Dietary Fiber-Deprived Gut Microbiota Degrades the Colonic Mucus Barrier and Enhances Pathogen Susceptibility [J].
Desai, Mahesh S. ;
Seekatz, Anna M. ;
Koropatkin, Nicole M. ;
Kamada, Nobuhiko ;
Hickey, Christina A. ;
Wolter, Mathis ;
Pudlo, Nicholas A. ;
Kitamoto, Sho ;
Terrapon, Nicolas ;
Muller, Arnaud ;
Young, Vincent B. ;
Henrissat, Bernard ;
Wilmes, Paul ;
Stappenbeck, Thaddeus S. ;
Nunez, Gabriel ;
Martens, Eric C. .
CELL, 2016, 167 (05) :1339-+
[8]   Leptin modulates the expression of secreted and membrane-associated mucins in colonic epithelial cells by targeting PKC, PI3K, and MAPK pathways [J].
El Homsi, Mahmoud ;
Ducroc, Robert ;
Claustre, Jean ;
Jourdan, Gerard ;
Gertler, Arieh ;
Estienne, Monique ;
Bado, Andre ;
Scoazec, Jean-Yves ;
Plaisancie, Pascale .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2007, 293 (01) :G365-G373
[9]   Hyper-osmolarity and calcium chelation: Effects on cystic fibrosis mucus [J].
Ermund, Anna ;
Meiss, Lauren N. ;
Gustafsson, Jenny K. ;
Hansson, Gunnar C. .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2015, 764 :109-117
[10]  
Escobar A, 2017, J ZOO WILDLIFE MED, V48, P380, DOI 10.1638/2016-0180r1.1
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