Characterization of Borrelia burgdorferi strains isolated from Korea by 16S rDNA sequence analysis and PCR-RFLP analysis of rrf (5S)-rrl (23S) intergenic spacer amplicons

被引：13
作者：
Lee, SH
Kim, BJ
Kim, JH
Park, KH
Yeo, SJ
Kim, SJ
Kook, YH [1 ]
机构：
[1] Seoul Natl Univ, Coll Med, Med Res Ctr, Dept Microbiol, Seoul 110799, South Korea
[2] Seoul Natl Univ, Coll Med, Med Res Ctr, Inst Endem Dis, Seoul 110799, South Korea
[3] Seoul Natl Univ Hosp, Clin Res Inst, Seoul 110799, South Korea
[4] Konkuk Univ, Coll Med, Dept Microbiol, Chonju 380701, Choongchungbuk, South Korea
[5] Pochun GHA Univ, Coll Med, Gen Hosp, Dept Pediat, Songnam 463670, Kyonggi Do, South Korea
来源：
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2000年 / 50卷
关键词：
Borrelia burgdorferi; 165; rDNA; rrf(5S)-rrl (23S) intergenic spacer gene; sequence analysis;
D O I：
10.1099/00207713-50-2-857
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Haenam strains of Borrelia burgdorferi, which had been isolated from Ixodes granulatus and Apodemus agrarius in Haenam, Korea, were characterized by PCR-RFLP analysis of rrf (55)-rrl (235) intergenic spacer amplicons and by sequence analysis of the 16S rRNA gene (rDNA), The MseI and DraI restriction patterns of the 55-23S intergenic spacer amplicons of Haenam strains differed from those of other B. burgdorferi sensu lato strains, Furthermore, in the phylogenetic tree based on the 16S rDNA sequences, Haenam strains formed a distinctive cluster, clearly separated from the other members of a. burgdorferi sensu late. These results suggest that, apart from Borrelia garinii and Borrelia afzelii, other genotypes of a, burgdorferi sensu late exist in Korea and the Haenam strain is a newly identified one.
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收藏
页码：857 / 863
页数：7
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