Clathrin-mediated endocytosis is involved in Tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector

被引:65
作者
Pan, Li-Long [1 ]
Chen, Qun-Fang [1 ]
Zhao, Juan-Juan [1 ]
Guo, Tao [1 ]
Wang, Xiao-Wei [1 ]
Hariton-Shalev, Aliza [2 ]
Czosnek, Henryk [2 ]
Liu, Shu-Sheng [1 ]
机构
[1] Zhejiang Univ, Inst Insect Sci, Minist Agr, Key Lab Agr Entomol, Hangzhou 310058, Zhejiang, Peoples R China
[2] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, Inst Plant Sci & Genet Agr, POB 10, IL-76100 Rehovot, Israel
基金
中国国家自然科学基金; 以色列科学基金会;
关键词
Bemisia tabaci; Clathrin-mediated endocytosis; Digestive tract; Tomato yellow leaf curl virus; Virus transmission; BEMISIA-TABACI; INSECT VECTOR; CIRCULATIVE TRANSMISSION; SELECTIVE BARRIER; SALIVARY-GLANDS; PATHWAY; BEGOMOVIRUSES; GEMINIVIRUS; CELLS; ENTRY;
D O I
10.1016/j.virol.2016.12.029
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci. The circulative translocation of the virus in the insect is known in its broad line. However, transit of TYLCV from the digestive tract into the haemolymph is poorly understood. We studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsRNA silencing. We monitored the quantities of TYLCV in the whitefly and virus transmission efficiency. Following endocytosis and endosome network disruption, the quantity of virus was higher in the midgut relative to that of the whole insect body, and the quantity of virus in the haemolymph was reduced. The transmission efficiency of TYLCV by the treated insects was also reduced. These findings indicate that clathrin-mediated endocytosis and endosomes play an important role in the transport of TYLCV across the whitey midgut.
引用
收藏
页码:152 / 159
页数:8
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