Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci. The circulative translocation of the virus in the insect is known in its broad line. However, transit of TYLCV from the digestive tract into the haemolymph is poorly understood. We studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsRNA silencing. We monitored the quantities of TYLCV in the whitefly and virus transmission efficiency. Following endocytosis and endosome network disruption, the quantity of virus was higher in the midgut relative to that of the whole insect body, and the quantity of virus in the haemolymph was reduced. The transmission efficiency of TYLCV by the treated insects was also reduced. These findings indicate that clathrin-mediated endocytosis and endosomes play an important role in the transport of TYLCV across the whitey midgut.
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Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USAOklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
de la Fuente, Jose
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Kocan, Katherine M.
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机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
Kocan, Katherine M.
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Almazan, Consuelo
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机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
Almazan, Consuelo
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Blouin, Edmour F.
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机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
机构:
Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Popovski, Smadar
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Kollenberg, Mario
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Leibniz Inst DSMZ German Collect Microorganisms &, Plant Virus Dept, Braunschweig, GermanyAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Kollenberg, Mario
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Gorovits, Rena
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Hebrew Univ Jerusalem, Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Gorovits, Rena
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Brown, Judith K.
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Univ Arizona, Sch Plant Sci, Tucson, AZ USAAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Brown, Judith K.
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Cicero, Joseph M.
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Univ Arizona, Sch Plant Sci, Tucson, AZ USAAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Cicero, Joseph M.
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Czosnek, Henryk
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Hebrew Univ Jerusalem, Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Czosnek, Henryk
;
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Winter, Stephan
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Ghanim, Murad
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Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
机构:
Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USAOklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
de la Fuente, Jose
;
Kocan, Katherine M.
论文数: 0引用数: 0
h-index: 0
机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
Kocan, Katherine M.
;
Almazan, Consuelo
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h-index: 0
机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
Almazan, Consuelo
;
Blouin, Edmour F.
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h-index: 0
机构:Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 USA
机构:
Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Popovski, Smadar
;
Kollenberg, Mario
论文数: 0引用数: 0
h-index: 0
机构:
Leibniz Inst DSMZ German Collect Microorganisms &, Plant Virus Dept, Braunschweig, GermanyAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Kollenberg, Mario
;
Gorovits, Rena
论文数: 0引用数: 0
h-index: 0
机构:
Hebrew Univ Jerusalem, Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Gorovits, Rena
;
Brown, Judith K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Sch Plant Sci, Tucson, AZ USAAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Brown, Judith K.
;
Cicero, Joseph M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Arizona, Sch Plant Sci, Tucson, AZ USAAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Cicero, Joseph M.
;
Czosnek, Henryk
论文数: 0引用数: 0
h-index: 0
机构:
Hebrew Univ Jerusalem, Inst Plant Sci & Genet Agr, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel
Czosnek, Henryk
;
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机构:
Winter, Stephan
;
Ghanim, Murad
论文数: 0引用数: 0
h-index: 0
机构:
Agr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, IsraelAgr Res Org, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel