The journal of eukaryotic microbiology

被引：54

作者：

Keller, AM ^{[1
]}

Cohen, J ^{[1
]}

机构：

[1] CNRS, Ctr Genet Mol, F-91198 Gif Sur Yvette, France

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2000年 / 47卷 / 01期

关键词：

complementation cloning; hybridization cloning;

D O I：

10.1111/j.1550-7408.2000.tb00001.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.

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页码：1 / 6

页数：6

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