Human adipose-derived mesenchymal stem cell spheroids improve recovery in a mouse model of elastase-induced emphysema

被引：32
作者：
Cho, Ryeon Jin [1 ]
Kim, You-Sun [1 ,2 ]
Kim, Ji-Young [2 ]
Oh, Yeon-Mok [1 ,2 ,3 ,4 ]
机构：
[1] Univ Ulsan, Coll Med, Seoul 05505, South Korea
[2] Asan Inst Life Sci, Seoul 05505, South Korea
[3] Asan Med Ctr, Dept Pulm & Crit Care Med, Seoul 05505, South Korea
[4] Asan Med Ctr, Clin Res Ctr Chron Obstruct Airway Dis, Seoul 05505, South Korea
来源：
BMB REPORTS | 2017年 / 50卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Adipose-derived mesenchymal stem cells; Chronic obstructive pulmonary disease; Regeneration; Spheroid cultures; HEPATOCYTE GROWTH-FACTOR; IN-VIVO; MATRIX METALLOPROTEINASES; ENDOTHELIAL-CELLS; STROMAL CELLS; LUNG; DISEASE; ANGIOGENESIS; STERNNESS; REPAIR;
D O I：
10.5483/BMBRep.2017.50.2.101
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema.
引用
收藏
页码：79 / 84
页数：6
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