Fructan metabolising enzymes in rhizophores of Vernonia herbacea upon excision of aerial organs

被引:32
作者
Asega, AF [1 ]
de Carvalho, MAM [1 ]
机构
[1] Secao Fisiol & Bioquim, Inst Bot, BR-01061970 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Asteraceae; cerrado; fructan metabolism; shoot excision; underground reserve organ; Vernonia herbacea;
D O I
10.1016/j.plaphy.2004.02.005
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The activities of fructan metabolising enzymes and fructan contents are reported for rhizophores of Vernonia herbacea (Veil.) Rusby induced to sprouting by shoot excision. The activities of fructan exohydrolase (1-FEH), sucrose: sucrose fructosyltransferase (1-SST), fructan: fructan fructosyltransferase (1-FFT) and invertase (INV) and the fructan contents were analysed every 3-4 days for 1 month by colorimetric and chromatographic methods. Sprouting of new shoots started on day 9. 1-FEH activity increased after day 13 and reached its maximum value 20 days after shoot excision. A gradual decrease in 1-SST activity was detected between days 3 and 9. 1-FFT activity exhibited fluctuations throughout the experimental period and a peak of activity for invertase was detected 9 days after shoot excision. Variation in fructan contents in vivo included a decrease until day 13 after which, levels remained practically unchanged. Fructan depolymerization and sprouting are concomitant processes in V herbacea and can be induced by shoot excision at any phenological phase. 1-FEH and 1-FFT seemed to act in a concerted way to catalyse fructan depolymerization, while 1-SST was inhibited, possibly due to interruption of sucrose supply to rhizophores from the aerial organs. (C) 2004 Elsevier SAS. All rights reserved.
引用
收藏
页码:313 / 319
页数:7
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