Tea polysaccharides from Camellia sinensis: chemical analysis, structural characterization, and inhibition of HeLa cells activity

被引：6
|
作者：
Gao, Yuefang [1 ]
Wang, Yancheng [2 ]
Ji, Xuening [1 ]
Xiao, Yao [3 ]
Xiao, Bin [1 ]
Peng, Pai [4 ]
机构：
[1] Northwest A&F Univ, Coll Hort, Yangling, Shaanxi, Peoples R China
[2] Hanshuiyun Tea Co Ltd, Ankang, Peoples R China
[3] Northwest A&F Univ, Dept Foreign Languages, Yangling, Shaanxi, Peoples R China
[4] Northwest A&F Univ, Coll Forestry, Yangling, Shaanxi, Peoples R China
来源：
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION | 2021年 / 26卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Tea polysaccharides; hydrothermal extraction; chemical analysis; structural characterization; HeLa cells;
D O I：
10.1080/1023666X.2021.1877957
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
Tea polysaccharides (TPS) were analyzed and characterized by HPAEC, GPC, FT-IR, AFM, HSQC, and HMBC NMR as well as methylation. The TPS mainly consisted of neutral (F-0, F-0.2, and F-0.3) and acidic polysaccharides (F-0.1) rich in glucuronic acid. Moreover, anti-proliferative activity of the TPS on HeLa cells was investigated. It was found that F-0 exhibited the highest inhibition rate (36.7%) with 200 mu g/ml treatment for 48 h. Structural characterization revealed that F-0 had a backbone chain of (1 -> 4)-beta-d-Glcp, (1 -> 6)-alpha-d-Galp, (1 -> 2)-alpha-l-Rhap and (1 -> 2,4)-alpha-l-Rhap residues with (1 -> 6)-alpha-d-Galp and acetyl group side chains.
引用
收藏
页码：240 / 252
页数：13
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