Isolation and Purification of Three Flavonoids from the Hawthorn Leaves by High Speed Countercurrent Chromatography, Combined with Isocratic Preparative Reversed-Phase High Performance Liquid Chromatography

被引:8
作者
Xu, Honglei [1 ]
Zhou, Tingting [1 ]
Wen, Jun [1 ]
Fan, Guorong [1 ]
Wu, Yutian [1 ]
机构
[1] Second Mil Med Univ, Sch Pharm, Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
关键词
2 ''-O-rhamnosylvitexin; 4 ''-O-glucosylvitexin; 4 '''-O-rhamnosylrutin; Countercurrent chromatography; Hawthorn leaves; Preparative chromatography; CRATAEGUS; EXTRACT; PHARMACOLOGY; TOXICOLOGY; PHARMACODYNAMICS; IDENTIFICATION; SEPARATION; GLYCOSIDES;
D O I
10.1080/10826070903163008
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Three flavonoids including 4'''-O-rhamnosylrutin, 2 ''-O-rhamnosylvitexin, and 4 ''-O-glucosylvitexin were isolated and purified from the hawthorn leaves by high speed countercurrent chromatography (HSCCC), combined together with isocratic preparative reversed-phase high performance liquid chromatography. Analytical HSCCC was used for the preliminary selection of a suitable solvent system composed of n-butanol-water (1:1, v/v). After two useful fractions were obtained by HSCCC using the optimal solvent system, one of them, fraction II, was further purified by reversed-phase semipreparative high performance liquid chromatography. From 500mg partially purified extract of the hawthorn leaves, 188.4mg 2 ''-O-rhamnosylvitexin, 28.5mg 4'''-O-rhamnosylrutin, and 55.3mg 4 ''-O-glucosylvitexin with purities of over 97%, respectively, were obtained. The structure identification of all pure fractions was carried out by UV, MS, H-1 NMR and C-13 NMR.
引用
收藏
页码:2216 / 2231
页数:16
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