Aqueous two-phase extraction of 1,3-propanediol from glycerol-based fermentation broths

被引：99

作者：

Li, Zhigang ^{[1
]}

Jiang, Bo ^{[1
]}

Zhang, Daijia ^{[1
]}

Xiu, Zhilong ^{[1
]}

机构：

[1] Dalian Univ Technol, Dept Biosci & Biotechnol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2009年 / 66卷 / 03期

关键词：

1,3-Propanediol; Aqueous two-phase extraction; Partition coefficient; Recovery; Fermentation; KLEBSIELLA-PNEUMONIAE; DOWNSTREAM SEPARATION; MICROBIAL-PRODUCTION; REACTIVE EXTRACTION; ZEOLITE MEMBRANE; PURIFICATION; GLUCOSE; PERVAPORATION; SYSTEM;

D O I：

10.1016/j.seppur.2009.02.009

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Separation of 1,3-propanediol from a fermentation broth is a difficult task that has become a bottleneck in industrial production. Aqueous two-phase systems composed of hydrophilic solvents and inorganic salts could be used to extract 1,3-propanediol from fermentation broth. The ethanol/ammonium sulfate system was investigated in detail, including phase diagram, effect of phase composition on partition, removal of cells and biomacromolecules from the broth. The results indicated that 1,3-propanediol took part in forming phases and had little effect on its partition. The highest partition coefficient (4.77) and recovery (93.7%) of 1,3-propanediol was obtained with an aqueous two-phase system composed of 46% (v/v) ethanol and saturated ammonium sulfate. At the same time the byproducts, 2,3-butandiol and acetoin, were also extracted with high efficiency. The maximum selective coefficient of 1,3-propanediol to glycerol was 6.0 in the experimental range. In addition, cells and proteins could be simultaneously removed from the fermentation broth, and the removal ratio for cells and proteins reached 99.7% and 79.0%, respectively. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：472 / 478

页数：7

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