Changes in Transcript and Protein Expression Levels in the Barley - Cochliobolus sativus Interaction

被引:4
作者
Jawhar, M. [1 ]
Shoaib, A. [1 ]
Arabi, M. I. E. [1 ]
Aldaoude, A. [1 ]
机构
[1] AECS, Dept Mol Biol & Biotechnol, POB 6091, Damascus, Syria
关键词
barley; Cochliobolus sativus; defense response; transcriptomics; proteomics; LOCI CONFERRING RESISTANCE; BIPOLARIS-SOROKINIANA; MESSENGER-RNA; GENES; GENOTYPES; PATHOGEN; IDENTIFICATION; ABUNDANCE;
D O I
10.1556/0806.44.2016.047
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Spot blotch, caused by Cochliobolus sativus, is an important barley disease which causes extensive grain yield losses worldwide. In order to investigate the molecular responses to the C. sativus infection, leaf transcriptome and proteome before and after fungus inoculation in a resistant barley genotype, were compared using cDNA-AFLP and 2-D PAGE techniques. A notable number of transcripts and proteins exhibiting significant differential accumulations were detected compared to the non-inoculated controls. Functional annotation of the transcripts and proteins revealed a wide range of pathways including cell wall fortification, metabolism, signal transduction and defence. Spearman correlations of the relative abundances for those genes represented by both an mRNA and a protein showed a weak (r(s) = 0.4; P < 0.001) relationship, indicating that post-transcriptional processes play a critical role in regulating the protein level during infection. Taken together, our study suggested that a joint analysis of the transcriptomic and proteomic of barley data can provide useful insights that may not be deciphered from individual analysis of mRNA or protein expressions.
引用
收藏
页码:104 / 113
页数:10
相关论文
共 26 条
[1]  
Al-Daoude A, 2013, J PLANT PATHOL, V95, P313
[2]  
Al-Daoune A, 2015, PHYTON-ANN REI BOT A, V55, P149
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]   Inheritance of partial resistance to spot blotch in barley [J].
Arabi, MIE .
PLANT BREEDING, 2005, 124 (06) :605-607
[5]   Identification of Cochliobolus sativus (spot blotch) isolates expressing differential virulence on barley genotypes in Syria [J].
Arabi, MIE ;
Jawhar, M .
JOURNAL OF PHYTOPATHOLOGY, 2004, 152 (8-9) :461-464
[6]   Gene expression profiling by cDNA-AFLP reveals potential candidate genes for partial resistance of 'President Roulin' against Venturia inaequalis [J].
Bastiaanse, Heloise ;
Muhovski, Yordan ;
Parisi, Olivier ;
Paris, Roberta ;
Mingeot, Dominique ;
Lateur, Marc .
BMC GENOMICS, 2014, 15
[7]   Molecular mapping of loci conferring resistance to different pathotypes of the spot blotch pathogen in barley [J].
Bilgic, H. ;
Steffenson, B. J. ;
Hayes, P. M. .
PHYTOPATHOLOGY, 2006, 96 (07) :699-708
[8]   Transcriptome analysis during cell division in plants [J].
Breyne, P ;
Dreesen, R ;
Vandepoele, K ;
De Veylder, L ;
Van Breusegem, F ;
Callewaert, L ;
Rombauts, S ;
Raes, J ;
Cannoot, B ;
Engler, G ;
Inzé, D ;
Zabeau, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14825-14830
[9]   The CC-NB-LRR-Type Rdg2a Resistance Gene Confers Immunity to the Seed-Borne Barley Leaf Stripe Pathogen in the Absence of Hypersensitive Cell Death [J].
Bulgarelli, Davide ;
Biselli, Chiara ;
Collins, Nicholas C. ;
Consonni, Gabriella ;
Stanca, Antonio M. ;
Schulze-Lefert, Paul ;
Vale, Giampiero .
PLOS ONE, 2010, 5 (09) :1-14
[10]   Differential dynamics of the mammalian mRNA and protein expression response to misfolding stress [J].
Cheng, Zhe ;
Teo, Guoshou ;
Krueger, Sabrina ;
Rock, Tara M. ;
Koh, Hiromi W. L. ;
Choi, Hyungwon ;
Vogel, Christine .
MOLECULAR SYSTEMS BIOLOGY, 2016, 12 (01)