Sensitive voltammetric sensor based on Isopropanol-Nafion-PSS-GR nanocomposite modified glassy carbon electrode for determination of Clenbuterol in pork

In the present study, poly(sodium 4-styrenesulfonate) (PSS) functionalized graphene (GR) was synthesised via a simple one-step chemical reduction of exfoliated graphite oxides in the presence of PSS. Characterisation of as-made nanocomposite using Fourier transform infrared spectroscopy (FT-IR) and ultraviolet and visible spectroscopy (UV-vis) clearly demonstrate the successful attachment of PSS to graphene sheets. A novel clenbuterol (CLB) electrochemical sensor was fabricated based on isopropanol-Nafion-PSS-GR composite film modified glassy carbon electrode. In the Britton-Robinson buffer (pH 1.2), the sensor exhibited superior electrocatalytic activity towards the oxidation of CLB. Applying linear sweep voltammetry, a good linear relationship of the oxidation peak current with respect to concentrations of CLB cross the range of 7.5 x 10(-8)-2.5 x 10(-5) mol L-1 and a detection limit of 2.2 x 10(-8) mol L-1 were achieved. The proposed method was successfully applied for the determination of CLB in pork. (C) 2014 Elsevier Ltd. All rights reserved.