Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici

被引：76

作者：

Chen, Cong ^{[1
]}

Wu, Wenhua ^{[1
]}

Xu, Xiaojuan ^{[1
]}

Zhang, Lina ^{[1
]}

Liu, Ying ^{[2
]}

Wang, Kaiping ^{[2
]}

机构：

[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China

[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 430030, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 105卷

基金：

中国国家自然科学基金;

关键词：

Carboxymethylated polysaccharide; Sulfated polysaccharide; Phosphated polysaccharide; Chain conformation; Anti-tumor activity; CHINESE HERBAL MEDICINES; PORIA-COCOS MYCELIA; AURICULARIA-AURICULA; HYPERBRANCHED POLYSACCHARIDE; GANODERMA-LUCIDUM; IN-VITRO; AGENTS; GLUCAN; SPECTRA; CELLS;

D O I：

10.1016/j.carbpol.2014.01.089

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A hyper branched (1 -> 4)-alpha-D-glucan (RPS3) with degree of branching of 35% extracted from Rhizoma Panacis Japonici adopted a sphere-like conformation and showed little bioactivity. Three derivatives including sulfated (S-), phosphated (P-) and carboxymethylated (CM-) RPS3 were then synthesized and characterized by FTIR, (CNMR)-C-13, and SEC-LLS-Vis-RI. As a result, the molecular weights of CM-RPS3, S-RPS3 and P-RPS3 decreased sharply in contrast to the original one, suggesting that chemical degradation has occurred. Moreover, the sphere-like conformation of RPS3 transferred into the random coil-like conformation according to the increased values of alpha(eta), alpha(s) and rho. It was ascribed to the occurrence of the preferential degradation for the branches. In vitro and in vivo tests demonstrated that the negatively charged S-RPS3 and P-RPS3 with properly low molecular mass significantly inhibited H-22 tumor cells growth. This work offered valuable results for broadening the biological applications of alpha-D-glucans. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：308 / 316

页数：9

共 54 条

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