Carbon nanodots featuring efficient FRET for two-photon photodynamic cancer therapy with a low fs laser power density

被引:134
作者
Wang, Jing [1 ,2 ,3 ]
Zhang, Zehui [4 ]
Zha, Shuai [1 ,2 ,3 ]
Zhu, Yinyan [1 ,2 ,3 ]
Wu, Peiyi [4 ]
Ehrenberg, Benjamin [5 ,6 ]
Chen, Ji-Yao [1 ,2 ,3 ]
机构
[1] Fudan Univ, State Key Lab Surface Phys, Shanghai 200433, Peoples R China
[2] Fudan Univ, Dept Phys, Shanghai 200433, Peoples R China
[3] Fudan Univ, Key Lab Micro & Nano Photon Struct, Minist Educ, Shanghai 200433, Peoples R China
[4] Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China
[5] Bar Ilan Univ, Dept Phys, IL-52900 Ramat Gan, Israel
[6] Bar Ilan Univ, Inst Nanotechnol, IL-52900 Ramat Gan, Israel
基金
中国国家自然科学基金;
关键词
Carbon dots; Two-photon absorption cross section; Fluorescence resonance energy transfer; Photodynamic therapy of cancers; SINGLET OXYGEN; CROSS-SECTIONS; COLLOIDAL CDSE; DOTS; NANOPARTICLES; ABSORPTION; CELLS; CYTOTOXICITY; FLUORESCENCE; EXCITATION;
D O I
10.1016/j.biomaterials.2014.07.063
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The 5,10,15,20-tetrakis(1-methyl 4-pyridinio) porphyrins (TMPyP), a photosensitizer used for photodynamic therapy of cancers (PDT), were linked to carbon dots (CDots) to form the conjugates of CDot-TMPyP by the electrostatic force. The 415 nm emission band of CDots was well overlapped with the absorption band of TMPyP, so that the Cdots in conjugates can work as donor to transfer the energy to TMPyP moiety by fluorescence resonance energy transfer (FRET) with an FRET efficiency of 45%, determined by the fluorescence lifetime change between the free CDots and conjugated CDots. The two-photon absorption cross section (TPACS) of TMPyP is as low as 110 GM and the TMPyP thus be not suitable for two-photon PDT. Whereas the CDots have high TPACS, and their TPACS are excitation wavelength dependent with the maximum value of 15000 GM at 700 nm. Therefore, the conjugates of CDot-TMPyP were explored for two-photon excitation (TPE) PDT. The two-photon image of CDot-TMPyP in Hela cells was clearly seen under the excitation of a 700 nm femto-second (fs) laser. The singlet oxygen production of CDot TMPyP was also much higher than that of TMPyP alone under TPE of a 700 nm fs laser. The in vitro PDT killing was further achieved with CDot-TMPyP by TPE of the 700 nm fs laser. Particularly herein the low power density of fs laser from unfocused laser beam was successfully used to carry out the TPE PDT, because of the high TPACS of CDots. These results demonstrate that the CDot-TMPyP conjugates are promising for TPE PDT and needed to investigate further. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:9372 / 9381
页数:10
相关论文
共 43 条
  • [1] Luminescent Carbon Nanodots: Emergent Nanolights
    Baker, Sheila N.
    Baker, Gary A.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (38) : 6726 - 6744
  • [2] Nanoparticles as vehicles for delivery of photodynamic therapy agents
    Bechet, Denise
    Couleaud, Pierre
    Frochot, Celine
    Viriot, Marie-Laure
    Guillemin, Francois
    Barberi-Heyob, Muriel
    [J]. TRENDS IN BIOTECHNOLOGY, 2008, 26 (11) : 612 - 621
  • [3] Nonlinear multiphoton processes in organic and polymeric materials
    Bhawalkar, JD
    He, GS
    Prasad, PN
    [J]. REPORTS ON PROGRESS IN PHYSICS, 1996, 59 (09) : 1041 - 1070
  • [4] Photosensitized production of singlet oxygen: spatially-resolved optical studies in single cells
    Breitenbach, Thomas
    Kuimova, Marina K.
    Gbur, Peter
    Hatz, Sonja
    Schack, Nickolass Bitsch
    Pedersen, Brian Wett
    Lambert, John D. C.
    Poulsen, Lars
    Ogilby, Peter R.
    [J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2009, 8 (04) : 442 - 452
  • [5] Carbon dots for multiphoton bioimaging
    Cao, Li
    Wang, Xin
    Meziani, Mohammed J.
    Lu, Fushen
    Wang, Haifang
    Luo, Pengju G.
    Lin, Yi
    Harruff, Barbara A.
    Veca, L. Monica
    Murray, Davoy
    Xie, Su-Yuan
    Sun, Ya-Ping
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (37) : 11318 - +
  • [6] The cytotoxicity of cadmium-based quantum dots
    Chen, Nan
    He, Yao
    Su, Yuanyuan
    Li, Xiaoming
    Huang, Qing
    Wang, Haifeng
    Zhang, Xiangzhi
    Tai, Renzhong
    Fan, Chunhai
    [J]. BIOMATERIALS, 2012, 33 (05) : 1238 - 1244
  • [7] Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334
  • [8] Photodynamic therapy for cancer
    Dolmans, DEJGJ
    Fukumura, D
    Jain, RK
    [J]. NATURE REVIEWS CANCER, 2003, 3 (05) : 380 - 387
  • [9] Carbon-Based Dots Co-doped with Nitrogen and Sulfur for High Quantum Yield and Excitation-Independent Emission
    Dong, Yongqiang
    Pang, Hongchang
    Yang, Hong Bin
    Guo, Chunxian
    Shao, Jingwei
    Chi, Yuwu
    Li, Chang Ming
    Yu, Ting
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2013, 52 (30) : 7800 - 7804
  • [10] Altruistic punishment in humans
    Fehr, E
    Gächter, S
    [J]. NATURE, 2002, 415 (6868) : 137 - 140