Tenascin-X promotes epithelial-to-mesenchymal transition by activating latent TGF-β

被引：74
作者：
Alcaraz, Lindsay B. [1 ,2 ,4 ,5 ,6 ]
Exposito, Jean-Yves [3 ,4 ,5 ]
Chuvin, Nicolas [1 ,2 ,4 ,5 ,6 ]
Pommier, Roxane M. [1 ,2 ,4 ,5 ,6 ]
Cluzel, Caroline [3 ,4 ,5 ]
Martel, Sylvie [1 ,2 ,4 ,5 ,6 ]
Sentis, Stephanie [1 ,2 ,4 ,5 ,6 ,7 ]
Bartholin, Laurent [1 ,2 ,4 ,5 ,6 ]
Lethias, Claire [3 ,4 ,5 ]
Valcourt, Ulrich [1 ,2 ,4 ,5 ,6 ]
机构：
[1] INSERM, Ctr Rech Cancerol Lyon, U1052, F-69000 Lyon, France
[2] CNRS, Ctr Rech Cancerol Lyon, UMR 5286, F-69000 Lyon, France
[3] Inst Biol & Chim Prot, FR3302, CNRS UMR 5305, F-69367 Lyon, France
[4] Univ Lyon, F-69000 Lyon, France
[5] Univ Lyon 1, F-69000 Lyon, France
[6] Ctr Leon Berard, F-69000 Lyon, France
[7] Fac Pharm Lyon, Inst Sci Pharmaceut & Biol, F-69000 Lyon, France
来源：
JOURNAL OF CELL BIOLOGY | 2014年 / 205卷 / 03期
关键词：
GROWTH-FACTOR-BETA; SMAD SIGNALING PATHWAY; EHLERS-DANLOS-SYNDROME; EXTRACELLULAR-MATRIX; BINDING-PROTEIN; ALPHA-11-BETA-1; INTEGRIN; INTERSTITIAL COLLAGENS; UP-REGULATION; IDENTIFICATION; TGF-BETA-1;
D O I：
10.1083/jcb.201308031
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
ransforming growth factor beta (TGF-beta) isoforms are secreted as inactive complexes formed through noncovalent interactions between the bioactive TGF-beta entity and its N-terminal latency-associated peptide prodomain. Extracellular activation of the latent TGF-beta complex is a crucial step in the regulation of TGF-beta function for tissue homeostasis. We show that the fibrinogen-like (FBG) domain of the matrix glycoprotein tenascin-X (TNX) interacts physically with the small latent TGF-beta complex in vitro and in vivo, thus regulating the bioavailabiliiy of mature TGF-beta to cells by activating the latent cytokine into an active molecule. Activation by the FBG domain most likely occurs through a conformational change in the latent complex and involves a novel cell adhesion dependent mechanism. We identify alpha 1 1 beta 1 integrin as a cell surface receptor for TNX and show that this integrin is crucial to elicit FBG-mediated activation of latent TGF-beta and subsequent epithelial-to-mesenchymal transition in mammary epithelial cells.
引用
收藏
页码：409 / 428
页数：20
相关论文
共 66 条
[1]   Specific processing of tenascin-C by the metalloprotease meprinβ neutralizes its inhibition of cell spreading [J].
Ambort, Daniel ;
Brellier, Florence ;
Becker-Pauly, Christoph ;
Stoecker, Walter ;
Andrejevic-Blant, Snezana ;
Chiquet, Matthias ;
Sterchi, Erwin E. .
MATRIX BIOLOGY, 2010, 29 (01) :31-42
[2]   Increased expression of integrin αvβ5 induces the myofibroblastic differentiation of dermal fibroblasts [J].
Asano, Y ;
Ihn, H ;
Yamane, K ;
Jinnin, M ;
Tamaki, K .
AMERICAN JOURNAL OF PATHOLOGY, 2006, 168 (02) :499-510
[3]   Involvement of αVβ5 integrin-mediated activation of latent transforming growth factor β1 in autocrine transforming growth factor β signaling in systemic sclerosis fibroblasts [J].
Asano, Y ;
Ihn, H ;
Yamane, K ;
Jinnin, M ;
Mimura, Y ;
Tamaki, K .
ARTHRITIS AND RHEUMATISM, 2005, 52 (09) :2897-2905
[4]   Physicochemical Activation of Recombinant Latent Transforming Growth Factor-beta's 1, 2, and 3 [J].
Brownh, Peter D. ;
Wakefiel, Lalage M. ;
Levinson, Arthur D. ;
Sporn, Michael B. .
GROWTH FACTORS, 1990, 3 (01) :35-43
[5]   Thrombospondin-1 is a major activator of TGF-β1 in vivo [J].
Crawford, SE ;
Stellmach, V ;
Murphy-Ullrich, JE ;
Ribeiro, SMF ;
Lawler, J ;
Hynes, RO ;
Boivin, GP ;
Bouck, N .
CELL, 1998, 93 (07) :1159-1170
[6]   Ligand-independent transforming growth factor-ß type I receptor signalling mediates type I collagen-induced epithelial-mesenchymal transition [J].
DeMaio, Lucas ;
Buckley, Stephen T. ;
Krishnaveni, Manda S. ;
Flodby, Per ;
Dubourd, Mickael ;
Banfalvi, Agnes ;
Xing, Yiming ;
Ehrhardt, Carsten ;
Minoo, Parviz ;
Zhou, Beiyun ;
Crandall, Edward D. ;
Borok, Zea .
JOURNAL OF PATHOLOGY, 2012, 226 (04) :633-644
[7]   Direct binding of Smad3 and Smad4 to critical TGFβ-inducible elements in the promoter of human plasminogen activator inhibitor-type 1 gene [J].
Dennler, S ;
Itoh, S ;
Vivien, D ;
ten Dijke, P ;
Huet, S ;
Gauthier, JM .
EMBO JOURNAL, 1998, 17 (11) :3091-3100
[8]   Identification and characterization of multiple species of tenascin-X in human serum [J].
Egging, D. F. ;
Peeters, A. C. T. M. ;
Grebenchtchikov, N. ;
Geurts-Moespot, A. ;
Sweep, C. G. J. ;
den Heijer, M. ;
Schalkwijk, J. .
FEBS JOURNAL, 2007, 274 (05) :1280-1289
[9]   Interactions of human tenascin-X domains with dermal extracellular matrix molecules [J].
Egging, David ;
van den Berkmortel, Franka ;
Taylor, Glen ;
Bristow, Jim ;
Schalkwijk, Joost .
ARCHIVES OF DERMATOLOGICAL RESEARCH, 2007, 298 (08) :389-396
[10]   Cell adhesion to tenascin-X - Mapping of cell adhesion sites and identification of integrin receptors [J].
Elefteriou, F ;
Exposito, JY ;
Garrone, R ;
Lethias, C .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 263 (03) :840-848
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