Genistein Induces G2/M Arrest in Gastric Cancer Cells by Increasing the Tumor Suppressor PTEN Expression

被引：42
作者：
Liu, Yan-Long [1 ]
Zhang, Guo-Qiang [2 ]
Yang, Yue [3 ]
Zhang, Chun-Yan [3 ]
Fu, Rong-Xia [4 ]
Yang, Yan-Mei [3 ]
机构：
[1] Harbin Med Univ, Dept Colorectal Surg, Affiliated Hosp 3, Harbin 150081, Heilongjiang, Peoples R China
[2] Harbin Med Univ, Dept Breast Surg, Affiliated Hosp 3, Harbin 150081, Heilongjiang, Peoples R China
[3] Harbin Med Univ, Canc Res Inst, Harbin 150081, Heilongjiang, Peoples R China
[4] Tianjin Agr Univ, Dept Food Sci, Biol Engn Lab, Tianjin, Peoples R China
来源：
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL | 2013年 / 65卷 / 07期
关键词：
ADENOVIRUS-MEDIATED TRANSFER; NF-KAPPA-B; CYCLE ARREST; CARCINOMA-CELLS; SIGNALING PATHWAY; DIETARY FACTORS; STOMACH-CANCER; BREAST-CANCER; UP-REGULATION; G(2)/M PHASE;
D O I：
10.1080/01635581.2013.810290
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Genistein, a major isoflavone found in soybeans, exhibits anticarcinogenic properties. The inhibitory effect of genistein on cell proliferation is associated with G2/M cell cycle arrest and inhibition of cdc2 activities. Here we assessed the role of PTEN in regulation of genistein-mediated G2/M cell cycle arrest in the gastric cancer cell lines (SGC-7901 and BGC-823). After 24h following treatment, genistein induced a concentration-dependent accumulation of cells in the G2/M phase of the cell cycle. The sustained G2/M arrest by genistein in SGC-7901 and BGC-823 cells is associated with increased phospho-cdc2 (Tyr15) and decreased cdc2 protein. Genistein treatment increased Wee1 levels and decreased phospho-Wee1 (Ser 642). Moreover, genistein substantially decreased the Ser473 and Thr308 phosphorylation of Akt and upregulated PTEN expression. Downregulation of PTEN by siRNA in genistein-treated cells increased phospho-Wee1 (Ser642), whereas decreased phospho-Cdc2 (Tyr15), resulting in decreased the G2/M cell cycle arrest. Therefore, induction of G2/M cell cycle arrest by genistein involved upregulation of PTEN.
引用
收藏
页码：1034 / 1041
页数：8
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