Analysis of peptides and amino acids by pre-column derivatization high performance liquid chromatography with fluorescence detection

被引：0

作者：

Liu, X ^{[1
]}

Xu, GL ^{[1
]}

Zhang, HS ^{[1
]}

Cheng, JK ^{[1
]}

机构：

[1] WUHAN UNIV,DEPT CHEM,WUHAN 430072,PEOPLES R CHINA

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 1997年 / 18卷 / 06期

关键词：

activated ester reagent; fluorescence derivatization; high performance liquid chromatography; peptides; amino acids;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new activated ester fluorescence derivatization reagent, N-hydroxysuccinimidyl-3-indolylacetate (SIIA) is used for analysis of small peptides and their hydrolysates by HPLC and fluorescence detection. In a one-step procedure, SIIA reacts with peptides and amino acids to form stable derivatives. Studies on derivatization conditions demonstrate excellent derivative properties cri the reagent, Maximal yields are observed with a molar reagent excess of approximately twenty five or greater and heated at 60 degrees C for 10 min in pH 8.0 buffer. SIIA derivatives of glutathionide, diglycine, glutamic acid, cystine and glycine are separated on C-18 reverse-column with the mobile phase of CH3OH/H2O(20/80, V/V) containing 10 mmol/L pH 3.6 citric acid-Na2HPO4 buffer. They are detected at lambda(ex)/lambda(em) = 278 nm/355 nm with the detection limits ranged from 0.2 pmol to 2.5 pmol.

引用

页码：873 / 876

页数：4

共 6 条

[1]

CHANG BY, 1995, CHINESE J ANAL CHEM, V23, P100

[2] SYNTHESIS OF A FLUORESCENT DERIVATIZING REAGENT, 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE, AND ITS APPLICATION FOR THE ANALYSIS OF HYDROLYSATE AMINO-ACIDS VIA HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].