Purification and characterization of the biological effects of phospholipase A2 from sea anemone Bunodosoma caissarum

被引：29

作者：

Martins, Rene D. ^{[2
]}

Alves, Renata S. ^{[2
]}

Martins, Alice M. C. ^{[3
]}

Barbosa, Paulo Sergio F. ^{[2
]}

Evangelista, Janaina S. A. M. ^{[4
]}

Evangelista, Joao Jose F. ^{[2
]}

Ximenes, Rafael M. ^{[2
]}

Toyama, Marcos H. ^{[5
]}

Toyama, Daniela O. ^{[6
]}

Souza, Alex Jardelino F. ^{[7
]}

Orts, Diego J. B. ^{[5
]}

Marangoni, Sergio ^{[7
]}

de Menezes, Dalgimar B. ^{[8
]}

Fonteles, Manasses C. ^{[6
]}

Monteiro, Helena S. A. ^{[1
,2
]}

机构：

[1] Univ Fed Ceara, Dept Fisiol & Farmacol, Fac Med, Inst Biomed, BR-60420970 Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Clin Res Unit, BR-60420970 Fortaleza, Ceara, Brazil

[3] Univ Fed Ceara, Dept Clin & Toxicol Anal, BR-60420970 Fortaleza, Ceara, Brazil

[4] Univ Estadual Ceara, Fac Vet, Fortaleza, Ceara, Brazil

[5] Univ Estadual Paulista, UNESP, Sao Vicente Unit, Sao Paulo, Brazil

[6] Univ Prebiteriana Mackenzie, Sao Paulo, Brazil

[7] Univ Estadual Campinas, Dept Biochem, IB, Sao Paulo, Brazil

[8] Univ Fed Ceara, Dept Pathol, BR-60420970 Fortaleza, Ceara, Brazil

来源：

TOXICON | 2009年 / 54卷 / 04期

关键词：

Bunodosoma caissarum; Phospholipase A(2); Biological effects; SNAKE-VENOM; BIOCHEMICAL-CHARACTERIZATION; STRUCTURAL-CHARACTERIZATION; NEURITE OUTGROWTH; RATTLESNAKE VENOM; COMPLEMENT-SYSTEM; NEMATOCYST VENOM; TOXIN; PEPTIDE; CLONING;

D O I：

10.1016/j.toxicon.2009.05.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Sea anemones contain a variety of biologically active substances. Bunodosoma caissarum is a sea anemone from the Cnidaria phylum, found only in Brazilian coastal waters. The aim of the present work was to study the biological effects Of PLA(2) isolated from the sea anemone B. caissarum on the isolated perfused kidney, the arteriolar mesenteric bed and on insulin secretion. Specimens of B. caissarum were collected from the Sao Vicente Channel on the southern coast of the State of Sao Paulo, Brazil. Reverse phase HPLC analysis of the crude extract of B. caissarum detected three PLA(2) proteins (named BcPLA(2)1, BCPLA(2)2 and BcPLA(2)3) found to be active in B. caissarum extracts. MALDI-TOF mass spectrometry of BcPLA(2)1 showed one main peak at 14.7 kDa. The N-terminal amino acid sequence of BcPLA(2)1 showed high amino acid sequence identity with PLA(2) group III protein isolated from the Mexican lizard (PA23 HELSU, HELSU, PA22 HELSU) and with the honey bee Apis mellifera (PLA(2) and 1POC_A). In addition, BcPLA(2)1 also showed significant overall homology to bee PLA(2). The enzymatic activity induced by native BCPLA(2)1 (20 mu g/well) was reduced by chemical treatment with p-bromophenacyl bromide (p-BPB) and with morin. BcPLA(2)1 strongly induced insulin secretion in presence of high glucose concentration. In isolated kidney, the PLA(2) from B. caissarum increased the perfusion pressure, renal vascular resistance, urinary flow, glomerular filtration rate, and sodium, potassium and chloride levels of excretion. BcPLA(2)1, however, did not increase the perfusion pressure on the mesenteric vascular bed. In conclusion, PLA(2), a group III phospholipase isolated from the sea anemone B. caissarum, exerted effects on renal function and induced insulin secretion in conditions of high glucose concentration. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：413 / 420

页数：8