Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)

被引:117
|
作者
Zha, Shenghua [1 ,2 ]
Zhao, Qingsheng [1 ]
Chen, Jinjin [1 ,2 ]
Wang, Liwei [1 ]
Zhang, Guifeng [1 ]
Zhang, Hong [3 ]
Zhao, Bing [1 ]
机构
[1] Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
[2] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
[3] Beijing Tong Ren Tang Hlth Pharmaceut Co Ltd, Beijing, Peoples R China
关键词
Maca; Lepidium meyenii; Polysaccharides; Monosaccharide composition; Antioxidant activity;
D O I
10.1016/j.carbpol.2014.05.017
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Water-soluble polysaccharides were separated from maca (Lepidium meyenii) aqueous extract (MAE). The crude polysaccharides were deproteinized by Sevag method. During the preparation process of maca polysaccharides, amylase and glucoamylase effectively removed starch in maca polysaccharides. Four Lepidium meyenii polysaccharides (LMPs) were obtained by changing the concentration of ethanol in the process of polysaccharide precipitation. All of the LMPs were composed of rhamnose, arabinose, glucose and galactose. Antioxidant activity tests revealed that LMP-60 showed good capability of scavenging hydroxyl free radical and superoxide radical at 2.0 mg/mL, the scavenging rate was 52.9% and 85.8%, respectively. Therefore, the results showed that maca polysaccharides had a high antioxidant activity and could be explored as the source of bioactive compounds. (C) 2014 Elsevier Ltd. All rights reserved.
引用
收藏
页码:584 / 587
页数:4
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