Direct electrochemistry of hemoglobin and biosensing for hydrogen peroxide using a film containing silver nanoparticles and poly(amidoamine) dendrimer

被引：64

作者：

Baccarin, Marina ^{[1
,2
]}

Janegitz, Bruno C. ^{[2
,3
]}

Berte, Rodrigo ^{[1
]}

Vicentini, Fernando Campanha ^{[2
]}

Banks, Craig E. ^{[4
]}

Fatibello-Filho, Orlando ^{[2
]}

Zucolotto, Valtencir ^{[1
]}

机构：

[1] Univ Sao Paulo, Inst Fis Sao Carlos, Nanomed & Nanotoxicol Grp, BR-13566390 Sao Carlos, SP, Brazil

[2] Univ Fed Sao Carlos, Dept Quim, BR-13565970 Sao Carlos, SP, Brazil

[3] Univ Fed Sao Carlos, Dept Ciencias Nat Matemat & Educ, BR-13600970 Araras, SP, Brazil

[4] Manchester Metropolitan Univ, Sch Chem & Environm, Div Chem & Environm Sci, Fac Sci & Engn, Manchester M1 5GD, Lancs, England

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2016年 / 58卷

基金：

巴西圣保罗研究基金会;

关键词：

Hemoglobin; Direct electron transfer; Third generation biosensor; Silver nanoparticles; Hydrogen peroxide; DIRECT ELECTRON-TRANSFER; FUNCTIONALIZED CARBON NANOTUBES; VOLTAMMETRIC DETERMINATION; PASTE ELECTRODE; COMPOSITE FILM; ELECTROCATALYSIS; CHITOSAN; GRAPHENE; TYROSINASE; SENSORS;

D O I：

10.1016/j.msec.2015.08.013

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A new architecture for a biosensor is proposed using a glassy carbon electrode (GCE) modified with hemoglobin (Hb) and silver nanopartides (AgNPs) encapsulated in poly(amidoamine) dendrimer (PAMAM). The biosensors were characterized using ultraviolet-visible spectroscopy, zeta-potential and cyclic voltammetry to investigate the interactions between Hb, AgNPs and the PAMAM film. The biosensor exhibited a well-defined cathodic peak attributed to reduction of the Fe3+ present in the heme group in Hb, as revealed by cyclic voltammetry in the presence of O-2. An apparent heterogeneous electron transfer rate of 4.1 s(-1) was obtained. The Hb-AgNPs-PAMAM/GCE third generation biosensor was applied in the amperometric determination of hydrogen peroxide over the linear range from 6.0 x 10(-6) to 9.1 x 10(-5) mol L-1 with a detection limit of 4.9 x 10(-6) mol L-1. The proposed method can be extended to immobilize and evaluate the direct electron transfer of other redox enzymes. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：97 / 102

页数：6

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