An Intact Urease Assembly Pathway Is Required To Compete with NikR for Nickel Ions in Helicobacter pylori

被引：35

作者：

Benanti, Erin L. ^{[1
]}

Chivers, Peter T. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA

来源：

JOURNAL OF BACTERIOLOGY | 2009年 / 191卷 / 07期

基金：

美国国家科学基金会;

关键词：

COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; IN-VIVO; MOLECULAR CHARACTERIZATION; GNOTOBIOTIC PIGLETS; PROTEINS HYPA; METAL-BINDING; HIGH-AFFINITY; DNA-BINDING; HYDROGENASE;

D O I：

10.1128/JB.01657-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We examined the effects of urease and hydrogenase assembly gene deletions on NikR activation in H. pylori strains 26695 and G27. The loss of any component of urease assembly increased NikR activity under Ni2+-limiting conditions, as measured by reduced transcript levels and Ni-63 accumulation. Additionally, SlyD functioned in urease assembly in strain 26695.

引用

页码：2405 / 2408

页数：4

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