Immunomodulatory effects of the methanolic extract from Pouteria campechiana leaves in macrophage functions

被引：19

作者：

Chan-Zapata, Ivan ^{[1
]}

Canul-Canche, Jaqueline ^{[1
]}

Fernandez-Martin, Karla ^{[1
]}

Martin-Quintal, Zhelmy ^{[2
]}

Cesar Torres-Romero, Julio ^{[3
]}

Cesar Lara-Riegos, Julio ^{[3
]}

Alberto Ramirez-Camacho, Mario ^{[4
]}

Ermilo Arana-Argaez, Victor ^{[1
]}

机构：

[1] Univ Autonoma Yucatan, Fac Quim, Lab Farmacol, Calle 43,613 X Calle 90, Col Inalambrica 97069, Merida, Mexico

[2] Univ Autonoma Yucatan, Fac Quim, Lab Quim Farmaceut, Merida, Mexico

[3] Univ Autonoma Yucatan, Fac Quim, Lab Bioquim & Genet Mol, Merida, Mexico

[4] Univ Autonoma Yucatan, Fac Quim, Ctr Informac Medicamentos, Merida, Mexico

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期

关键词：

Pouteria campechiana; macrophage; immunomodulation; cytotoxic; ANTIOXIDANT ACTIVITIES;

D O I：

10.1080/09540105.2017.1386163

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The present study aimed to examine the immunomodulatory properties of the methanolic (MeOH) extract from Pouteria. campechiana leaves in peritoneal macrophages of Balb/c mice. Peritoneal macrophages isolated from mice and Vero cells were treated with the MeOH extract from leaves. Cell viability of the macrophages and Vero cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. The phagocytic activity, as nitric oxide (NO), hydrogen peroxide (H2O2), interleukin 6 (IL-6) and tumour necrosis factor (TNF-) production were evaluated on peritoneal macrophages. Results showed that the MeOH extract from leaves was able to stimulate the phagocytic activity and increase NO, H2O2 and cytokines production. The viability assays do not show cytotoxic effect on cell viability and cause a significative proliferative effect in the macrophages of a concentration-dependent manner. These results conclude that the MeOH extract from P.campechiana leaves possessed a stronger immunostimulatory effect in a concentration-dependent manner without affect the cell viability.

引用

页码：386 / 399

页数：14