Detection and infectivity potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) environmental contamination in isolation units and quarantine facilities

被引：82

作者：

Ben-Shmuel, Amir ^{[1
]}

Brosh-Nissimov, Tal ^{[3
,4
]}

Glinert, Itai ^{[1
]}

Bar-David, Elad ^{[1
]}

Sittner, Assa ^{[1
]}

Poni, Reut ^{[1
]}

Cohen, Regev ^{[5
]}

Achdout, Hagit ^{[1
]}

Tamir, Hadas ^{[1
]}

Yahalom-Ronen, Yfat ^{[1
]}

Politi, Boaz ^{[1
]}

Melamed, Sharon ^{[1
]}

Vitner, Einat ^{[1
]}

Cherry, Lilach ^{[1
]}

Israeli, Ofir ^{[2
]}

Beth-Din, Adi ^{[2
]}

Paran, Nir ^{[1
]}

Israely, Tomer ^{[1
]}

Yitzhaki, Shmuel ^{[1
]}

Levy, Haim ^{[1
]}

Weiss, Shay ^{[1
]}

机构：

[1] Israel Inst Biol Res, Dept Infect Dis, 24th 329 Reuven Lerer St, Ness Ziona, Israel

[2] Israel Inst Biol Res, Dept Biochem & Mol Genet, Ness Ziona, Israel

[3] Assuta Ashdod Univ Hosp, Infect Dis Unit, Ashdod, Israel

[4] Ben Gurion Univ Negev, Fac Hlth Sci, Beer Sheva, Israel

[5] Laniado Hosp, Infect Dis Unit, Netanya, Israel

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2020年 / 26卷 / 12期

关键词：

Contamination; Coronavirus; COVID-19; SARS-CoV-2; Surface; Viability; TRANSMISSION; OUTBREAK; VIRUS; SARS;

D O I：

10.1016/j.cmi.2020.09.004

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Objectives: Environmental surfaces have been suggested as likely contributors in the transmission of COVID-19. This study assessed the infectivity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminating surfaces and objects in two hospital isolation units and a quarantine hotel. Methods: SARS-CoV-2 virus stability and infectivity on non-porous surfaces was tested under controlled laboratory conditions. Surface and air sampling were conducted at two COVID-19 isolation units and in a quarantine hotel. Viral RNA was detected by RT-PCR and infectivity was assessed by VERO E6 CPE test. Results: In laboratory-controlled conditions, SARS-CoV-2 gradually lost its infectivity completely by day 4 at ambient temperature, and the decay rate of viral viability on surfaces directly correlated with increase in temperature. Viral RNA was detected in 29/55 surface samples (52.7%) and 16/42 surface samples (38%) from the surroundings of symptomatic COVID-19 patients in isolation units of two hospitals and in a quarantine hotel for asymptomatic and very mild COVID-19 patients. None of the surface and air samples from the three sites (0/97) were found to contain infectious titres of SARS-Cov-2 on tissue culture assay. Conclusions: Despite prolonged viability of SARS-CoV-2 under laboratory-controlled conditions, uncultivable viral contamination of inanimate surfaces might suggest low feasibility for indirect fomite transmission. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of European Society of Clinical Microbiology and Infectious Diseases.

引用

页码：1658 / 1662

页数：5

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