Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions

被引：18

作者：

Ren, Gang ^{[1
,2
]}

Wang, Yan ^{[1
]}

Qin, Jianguang ^{[3
]}

Tang, Jinyu ^{[1
]}

Zheng, Xiafei ^{[1
]}

Li, Youming ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Shaoxing Univ, Coll Life Sci, Shaoxing 312000, Peoples R China

[3] Flinders Univ S Australia, Sch Biol Sci, Adelaide, SA 5001, Australia

来源：

GENE | 2014年 / 546卷 / 01期

关键词：

Hyriopsis cumingii; Carbonic anhydrase; Expression; Shell growth; pH homeostasis; PHYSIOLOGICAL-RESPONSES; LYMNAEA-STAGNALIS; CALCIUM-BINDING; ANODONTA-CYGNEA; SHELL FORMATION; MANTLE; CALCIFICATION; PROTEINS; CA2+; GASTROPODA;

D O I：

10.1016/j.gene.2014.05.039

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Gene encoding for alpha-carbonic anhydrases (alpha-CAs) and their functions in fundamental metabolism and biomineralization are widely identified in mollusks. However, the transcriptional regulation of alpha-CA genes in response to various environmental conditions remains unknown. In the present study, we characterized a cDNA encoding for an alpha-CA (HcCA) from the freshwater pearl mussel Hyriopsis cumingii. The spatial and temporal expression patterns of HcCA indicate that this gene is mainly expressed in the mantle of juvenile mussels. The expression profile of HcCA under various environmental conditions reveals that the transcription of HcCA is significantly regulated by Ca2+ concentration, water temperature, pH and air exposure. Our results suggest that HcCA is a crucial target gene by which the external environmental conditions affecting shell growth and pH homeostasis of H. cumingii. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：56 / 62

页数：7

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