Membrane supported bilayer lipid membranes array: preparation, stability and ion-channel insertion

被引：40
|
作者：
Favero, G
D'Annibale, A
Campanella, L
Santucci, R
Ferri, T
机构：
[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy
[2] Univ Roma Tor Vergata, Dipartimento Med Sperimentale & Sci Biochim, I-00133 Rome, Italy
来源：
ANALYTICA CHIMICA ACTA | 2002年 / 460卷 / 01期
关键词：
reconstituted biological membranes; bilayer lipid membranes; biosensors; ion channels; ion carriers; Valinomycine; Gramicidin;
D O I：
10.1016/S0003-2670(02)00139-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In the present paper we describe the preparation and characterization of reconstituted bilayer lipid membranes (BLM) supported on a polycarbonate membrane. BLMs self-assemble on the holes of the membrane, which was previously covered with An and an octadecanethiol (ODT) self-assembled monolayer (SAM). The obtained mixed hybrid bilayer lipid membranes (MHBLM) array allows recording of relatively intense signals. The steps leading to formation of the system have been investigated, its stability determined and reproducibility evaluated. The insertion of an ion channel (Gramicidin D) and a K+ carrier (Valinomycine) was also evaluated. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：23 / 34
页数：12
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