Expression of neuropeptide Y and pro-opiomelanocortin in hypothalamic arcuate nucleus in 17α-ethinyl estradiol-induced intrahepatic cholestasis pregnant rat offspring

被引：8
|
作者：
Shi, Qingyun [1 ]
Wang, Jingjing [2 ]
Yan, Shi [3 ]
Zhao, Jin [1 ]
Li, Hongxia [1 ]
机构：
[1] Capital Med Univ, Beijing Shi Jitan Hosp, Dept Obstet & Gynecol, Beijing 100038, Peoples R China
[2] Capital Med Univ, Dept Lab Anim Sci, Beijing 100038, Peoples R China
[3] Sichuan Univ, West China Med Sch, Dept Clin Med, Chengdu, Peoples R China
来源：
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH | 2014年 / 40卷 / 02期
基金：
中国国家自然科学基金;
关键词：
arcuate nucleus; intrahepatic cholestasis of pregnancy; neuropeptides; rat; UTEROPLACENTAL INSUFFICIENCY; DEVELOPMENTAL ORIGINS; ENERGY-BALANCE; ADULT DISEASE; FETAL ORIGINS; FOOD-INTAKE; IN-UTERO; OBESITY; NEURONS; EXPOSURE;
D O I：
10.1111/jog.12206
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
AimThe purpose of this study was to investigate the expression of neuropeptide Y (NPY) and pro-opiomelanocortin (POMC) in the hypothalamic arcuate nucleus of intrahepatic cholestasis pregnant (ICP) offspring. MethodsThe model of ICP rats was established by injecting s.c. 17-ethinyl estradiol. The expression of NPY and POMC in female offspring was determined by quantitative real-time reverse transcription polymerase chain reaction, western blotting and immunohistochemistry at birthday and 6months. ResultsICP group offspring had lower bodyweight at birthday. ICP offspring were markedly heavier than control offspring after 6months. mRNA and protein expression of NPY and POMC significantly increased at 6months as compared with the birthday among control offspring. Among ICP offspring, mRNA and protein expression of NPY and POMC also were higher at 6months than at birthday. The mRNA and protein expression of NPY were higher in ICP offspring than that of control offspring at birthday. The mRNA and protein expression of POMC were decreased in ICP offspring than that of control offspring. After 6months, the mRNA expression and protein expression of NPY also were higher in ICP offspring than that of control offspring. The mRNA expression and protein expression of POMC also were decreased in ICP offspring than that of control offspring. The results were confirmed by immunohistochemistry. ConclusionICP offspring demonstrated evidence of persistent appetite stimulation with significantly upregulated NPY expression and reduced POMC expression at birthday and 6months. ICP offspring showed a hunger state and then gained weight.
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收藏
页码：445 / 452
页数：8
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