Structural characterization and anti-inflammatory activity of alkali-soluble polysaccharides from purple sweet potato

被引：75

作者：

Chen, Hong ^{[1
]}

Sun, Jian ^{[2
,3
]}

Liu, Jun ^{[1
]}

Gou, Yarun ^{[1
]}

Zhang, Xin ^{[1
]}

Wu, Xiaonan ^{[1
]}

Sun, Rui ^{[1
]}

Tang, Sixue ^{[1
]}

Kan, Juan ^{[1
]}

Qian, Chunlu ^{[1
]}

Zhang, Nianfeng ^{[1
]}

Jin, Changhai ^{[1
]}

机构：

[1] Yangzhou Univ, Coll Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China

[2] Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China

[3] Xuzhou Inst Agr Sci Jiangsu, Xuhuai Area, Xuzhou 221131, Jiangsu, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2019年 / 131卷

基金：

中国国家自然科学基金;

关键词：

Alkali-soluble polysaccharide; Anti-inflammation activity; Structural characterization; Purple sweet potato; VITRO ANTIOXIDANT ACTIVITY; NF-KAPPA-B; IN-VITRO; SULFATED POLYSACCHARIDE; SARGASSUM-HORNERI; PURIFICATION; ACTIVATION; EXTRACTION; INJURY;

D O I：

10.1016/j.ijbiomac.2019.03.126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, the structural characterization and anti-inflammation effect of dilute alkali-soluble polysaccharides from purple sweet potato were investigated. Three fractions (F-1, F-2 and F-3) were obtained by purifying crude polysaccharides on DEAE-52 cellulose column. The main fraction (F-1) was further purified on Sephadex G-200 column to afford purified alkali-soluble sweet potato polysaccharide (ASPP). The chemical structure of ASPP was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis and nuclear magnetic resonance spectroscopy. Monosaccharide compositional analysis showed ASPP was composed of rhamnose, arabinose, xylose, mannose and glucose in the molar ratio of 2.8:1.9:1.0:7.6:533. Moreover, the backbone of ASPP was composed of 1,4-linked Glcp with side chains attached to the O-6 position. The anti inflammation effect of ASPP was further investigated by in vitro and in vivo experiments. Results showed ASPP could inhibit the levels of nitric oxide, interleukin (IL)-6, IL-1 beta and TNF-alpha but increase the production of IL-10 in lipopolysaccharide (LPS)-treated RAW 264.7 macrophage cells. In addition ASPP could reduce the secretion of IL-6, IL-1 beta and TNF-alpha in LPS-treated mice. Our results suggest ASPP can be developed as a novel anti-inflammation agent. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：484 / 494

页数：11

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