Bacterial Endophyte Sphingomonas sp LK11 Produces Gibberellins and IAA and Promotes Tomato Plant Growth

被引：314

作者：

Khan, Abdul Latif ^{[1
]}

Waqas, Muhammad ^{[2
]}

Kang, Sang-Mo ^{[2
]}

Al-Harrasi, Ahmed ^{[1
]}

Hussain, Javid ^{[1
]}

Al-Rawahi, Ahmed ^{[1
]}

Al-Khiziri, Salima ^{[1
]}

Ullah, Ihsan ^{[2
]}

Ali, Liaqat ^{[1
]}

Jung, Hee-Young ^{[2
]}

Lee, In-Jung ^{[2
]}

机构：

[1] Univ Nizwa, Dept Biol Sci & Chem, Nizwa, Oman

[2] Kyungpook Natl Univ, Sch Appl Biosci, Taegu 702701, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2014年 / 52卷 / 08期

关键词：

Solanum lycopersicum; Sphingomonas sp LK11; endophytism; gibberellins; indole acetic acid; IDENTIFICATION; DIAZOTROPHICUS; BIOSYNTHESIS; MULTIPLE; ACID;

D O I：

10.1007/s12275-014-4002-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Plant growth promoting endophytic bacteria have been identified as potential growth regulators of crops. Endophytic bacterium, Sphingomonas sp. LK11, was isolated from the leaves of Tephrosia apollinea. The pure culture of Sphingomonas sp. LK11 was subjected to advance chromatographic and spectroscopic techniques to extract and isolate gibberellins (GAs). Deuterated standards of [17, 17-H-2(2)]-GA(4), [17, 17-H-2(2)]-GA(9) and [17, 17-H-2(2)]-GA(20) were used to quantify the bacterial GAs. The analysis of the culture broth of Sphingomonas sp. LK11 revealed the existence of physiologically active gibberellins (GA(4): 2.97 +/- 0.11 ng/ml) and inactive GA(9) (0.98 +/- 0.15 ng/ml) and GA(20) (2.41 +/- 0.23). The endophyte also produced indole acetic acid (11.23 +/- 0.93 mu M/ml). Tomato plants inoculated with endophytic Sphingomonas sp. LK11 showed significantly increased growth attributes (shoot length, chlorophyll contents, shoot, and root dry weights) compared to the control. This indicated that such phyto-hormones-producing strains could help in increasing crop growth.

引用

页码：689 / 695

页数：7

共 59 条

[11] Production of indole-3-acetic acid and gibberellins A1 and A3 by Acetobacter diazotrophicus and Herbaspirillum seropedicae in chemically-defined culture media [J].