Epidemic multidrug-resistant Acinetobacter baumannii related to European clonal types I and II in Rome (Italy)

被引：42
作者：
D'Arezzo, S. [2 ]
Capone, A. [2 ]
Petrosillo, N. [2 ]
Visca, P. [1 ,2 ]
机构：
[1] Univ Roma Tre, Dipartimento Biol, I-00146 Rome, Italy
[2] IRCCS, Natl Inst Infect Dis Lazzaro Spallanzani, Rome, Italy
来源：
CLINICAL MICROBIOLOGY AND INFECTION | 2009年 / 15卷 / 04期
关键词：
Acinetobacter baumannii; epidemiology; integrons; multidrug resistance; nosocomial infection; typing; INTENSIVE-CARE-UNIT; HYDROLYZING OXACILLINASE OXA-58; FIELD GEL-ELECTROPHORESIS; MOLECULAR CHARACTERIZATION; PSEUDOMONAS-AERUGINOSA; MULTIPLEX PCR; STRAINS; IDENTIFICATION; OUTBREAK; HOSPITALS;
D O I：
10.1111/j.1469-0691.2009.02668.x
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The molecular epidemiology and the genetic basis of antibiotic resistance in 88 multidrug-resistant (MDR) Acinetobacter baumannii strains isolated during 18 months from infected patients in seven intensive care units (ICUs) in Rome were investigated. Random amplified polymorphic DNA and macrorestriction analysis identified two predominant clonal types, genetically related to the European epidemic clones I (type 2) and II (type 1), accounting for 98.9% of A. baumannii ICU isolates. Type 1 was isolated from all ICUs under survey. Class 1 integrons of 2.2 and 2.5 kb were detected in type 1 and type 2 isolates, respectively. The integron structures were similar to those previously determined for epidemic A. baumannii strains from various European countries, and suggestive of integron rearrangement/exchange among isolates related to the European epidemic clones I and II. Carbapenem resistance was associated with the presence of the bla(OXA-58) gene in type 1 isolates. The results indicate that the A. baumannii type 1 clone has a high potential of spreading among hospitals.
引用
收藏
页码：347 / 357
页数：11
相关论文
共 45 条
  • [1] Multidrug-resistant Acinetobacter baumannii
    Abbo, A
    Navon-Venezia, S
    Orly, HM
    Krichali, T
    Siegman-Igra, Y
    Carmeli, Y
    [J]. EMERGING INFECTIOUS DISEASES, 2005, 11 (01) : 22 - 29
  • [2] Alert surveillance of intensive care unit-acquired Acinetobacter infections in a Sicilian hospital
    Agodi, A
    Zarrilli, R
    Barchitta, M
    Anzaldi, A
    Di Popolo, A
    Mattaliano, A
    Ghiraldi, E
    Travali, S
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (03) : 241 - 247
  • [3] [Anonymous], 1989, Molecular Cloning: A Laboratory Manual
  • [4] First report of the carbapenem-hydrolyzing oxacillinase OXA-58 in Acinetobacter baumannii isolates in Italy
    Bertini, Alessia
    Giordano, Alessandra
    Varesi, Paola
    Villa, Laura
    Mancini, Carlo
    Carattoli, Alessandra
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (06) : 2268 - 2269
  • [5] Molecular surveillance of European quinolone-resistant clinical isolates of Pseudomonas aeruginosa and Acinetobacter spp. using automated ribotyping
    Brisse, S
    Milatovic, S
    Fluit, AC
    Kusters, KA
    Toelstra, A
    Verhoef, J
    Schmitz, F
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (10) : 3636 - 3645
  • [6] RAPD-PCR typing of Acinetobacter isolates from activated sludge systems designed to remove phosphorus microbiologically
    Carr, E
    Eason, H
    Feng, S
    Hoogenraad, A
    Croome, R
    Soddell, J
    Lindrea, K
    Seviour, R
    [J]. JOURNAL OF APPLIED MICROBIOLOGY, 2001, 90 (03) : 309 - 319
  • [7] Clinical and Laboratory Standards Institute, 2007, M100S17 CLSI
  • [8] Occurrence of carbapenern-resistant Acinetobacter baumannii clones at multiple hospitals in London and southeast England
    Coelho, Juliana M.
    Turton, Jane F.
    Kaufmann, Mary E.
    Glover, Judith
    Woodford, Neil
    Warner, Marina
    Palepou, Marie-France
    Pike, Rachel
    Pitt, Tyrone L.
    Patel, Bharat C.
    Livermore, David M.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (10) : 3623 - 3627
  • [9] Efficacy of sulbactam alone and in combination with ampicillin in nosocomial infections caused by multiresistant Acinetobacter baumannii
    Corbella, X
    Ariza, J
    Ardanuy, C
    Vuelta, M
    Tubau, F
    Sora, M
    Pujol, M
    Gudiol, F
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1998, 42 (06) : 793 - 802
  • [10] Identification of widespread, closely related Acinetobacter baumannii isolates in Portugal as a subgroup of European clone II
    Da Silva, G.
    Dijkshoorn, L.
    van der Reijden, T.
    van Strijen, B.
    Duarte, A.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (02) : 190 - 195
12345