Magnetic microbead-based electrochemical immunoassays

被引:65
作者
Kuramitz, Hideki [1 ]
机构
[1] Toyama Univ, Grad Sch Sci & Engn Res, Dept Environm Biol & Chem, Toyama 9308555, Japan
关键词
Electrochemical immunoassay; Magnetic microbead; Enzyme label; Nanoparticle tag; BEAD-BASED IMMUNOASSAY; ROTATING-DISK ELECTRODE; WHEAT-GERM-AGGLUTININ; DNA HYBRIDIZATION; VOLTAMMETRIC EVALUATION; AMPEROMETRIC DETECTION; QUANTUM-DOT; ASSAY; NANOPARTICLES; COMPLEXES;
D O I
10.1007/s00216-009-2650-y
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This review provides a summary of recent works concerning electrochemical immunoassays using magnetic microbeads as a solid phase. Recent research activity has led to innovative and powerful detection strategies that have been resulted in sensitive electrochemical detection. Coupling of magnetic microbeads with highly sensitive electrochemical detection provides a useful analytical method for environmental evaluation and clinical diagnostics, etc. The huge surface area and high dispersion capability of magnetic microbeads strongly contributes towards the development of new sensitive, rapid, user-friendly, and miniaturized electrochemical immunoassay systems. Moreover, the immunocomplexes formed on the magnetic microbead surface can be easily detected without pretreatment steps such as preconcentration or purification, which are normally required for standard methods. The discussion in this review is organized in two main subjects that include magnetic-microbead-based assays using enzyme labels and nanoparticle tags.
引用
收藏
页码:61 / 69
页数:9
相关论文
共 55 条
[31]   Flow injection immunoanalysis based on a magnetoimmunosensor system [J].
Solé, S ;
Alegret, S ;
Céspedes, F ;
Fàbregas, E ;
Díez-Caballero, T .
ANALYTICAL CHEMISTRY, 1998, 70 (08) :1462-1467
[32]   Voltammetric evaluation of the binding between wheat germ agglutinin and thionine/glucose-modified magnetic microbeads [J].
Sugawara, Kazuharu ;
Senbongi, Atsushi ;
Kamiya, Naoto ;
Hirabayashi, George ;
Kuramitz, Hideki .
ANALYTICAL SCIENCES, 2008, 24 (06) :717-720
[33]   Voltammetric evaluation for the binding of wheat germ agglutinin to glucosamine-modified magnetic microbead [J].
Sugawara, Kazuharu ;
Kamiya, Naoto ;
Hirabayashi, George ;
Kuramitz, Hideki .
TALANTA, 2007, 72 (03) :1123-1128
[34]   Fully automated chemiluminescence immunoassay of insulin using antibody-protein A-bacterial magnetic particle complexes [J].
Tanaka, T ;
Matsunaga, T .
ANALYTICAL CHEMISTRY, 2000, 72 (15) :3518-3522
[35]   Ultrasensitive electrochemical immunosensor for clinical immunoassay using thionine-doped magnetic gold nanospheres as labels and horseradish peroxidase as enhancer [J].
Tang, Dianping ;
Yuan, Ruo ;
Chal, Yaqin .
ANALYTICAL CHEMISTRY, 2008, 80 (05) :1582-1588
[36]   Bead-based electrochemical immunoassay for bacteriophage MS2 [J].
Thomas, JH ;
Kim, SK ;
Hesketh, PJ ;
Halsall, HB ;
Heineman, WR .
ANALYTICAL CHEMISTRY, 2004, 76 (10) :2700-2707
[37]   Microdrop analysis of a bead-based immunoassay [J].
Thomas, JH ;
Ronkainen-Matsuno, NJ ;
Farrell, S ;
Halsall, HB ;
Heineman, WR .
MICROCHEMICAL JOURNAL, 2003, 74 (03) :267-276
[38]  
TRELOAR P, 1997, PRINCIPLES PRACTICE, P483
[39]   IMMUNOASSAY USING ANTIGEN-ENZYME CONJUGATES [J].
VANWEEME.BK ;
SCHUURS, AHW .
FEBS LETTERS, 1971, 15 (03) :232-&
[40]   A NEW TECHNIQUE OF ENZYME ENTRAPMENT FOR PLANAR BIOSENSORS [J].
VARLAN, AR ;
SULS, J ;
JACOBS, P ;
SANSEN, W .
BIOSENSORS & BIOELECTRONICS, 1995, 10 (08) :R15-R19