Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer

被引：37
作者：
Chen, Dong-liang [1 ,2 ]
Wang, Zhi-qiang [1 ,2 ]
Ren, Chao [1 ,2 ]
Zeng, Zhao-lei [1 ,3 ]
Wang, De-shen [1 ,2 ]
Luo, Hui-yan [1 ,2 ]
Wang, Feng [1 ,2 ]
Qiu, Miao-zhen [1 ,2 ]
Bai, Long [1 ,2 ]
Zhang, Dong-sheng [1 ,2 ]
Wang, Feng-hua [1 ,2 ]
Li, Yu-hong [1 ,2 ]
Xu, Rui-hua [1 ,2 ]
机构：
[1] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Guangzhou 510060, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Ctr Canc, State Key Lab Oncol South China, Dept Med Oncol, Guangzhou 510060, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Ctr Canc, Dept Expt Res, Guangzhou 510060, Guangdong, Peoples R China
来源：
JOURNAL OF TRANSLATIONAL MEDICINE | 2013年 / 11卷
关键词：
Gastric cancer; Paxillin; Tumor progression; Prognosis; CELL LUNG-CANCER; TYROSINE PHOSPHORYLATION; ADHESION; METASTASIS; GROWTH; ADENOCARCINOMA; PROLIFERATION; MUTATIONS; INVASION; PATHWAY;
D O I：
10.1186/1479-5876-11-277
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Background: Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear. Methods: The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated. Results: PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P = 0.001) and advanced tumor stage (P = 0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P < 0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P = 0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells. Conclusions: PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer.
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页数：9
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