Determination of bacteriorhodopsin orientation in monolayers by infrared spectroscopy

被引：13
作者：
Methot, M
Boucher, F
Salesse, C
Subirade, M
Pezolet, M
机构：
[1] UNIV QUEBEC, CTR RECH PHOTOBIOPHYS, TROIS RIVIERES, PQ G9A 5H7, CANADA
[2] UNIV LAVAL, DEPT CHIM, CTR RECH SCI & INGN MACROMOL, ST FOY, PQ G1K 7P4, CANADA
来源：
THIN SOLID FILMS | 1996年 / 284卷
关键词：
bacteriorhodopsin; monolayers; Langmuir-Blodgett films; FTIR-ATR spectroscopy;
D O I：
10.1016/S0040-6090(95)08407-X
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
In the present study, Fourier transform infrared-attenuated total reflection spectroscopy has been used to characterize Langmuir-Blodgett films of purple membranes deposited on Ge crystals at different surface pressures. The results obtained indicate that the average angle of the protein's cr-helices with respect to the interface normal remains unchanged with increasing lateral pressure. Moreover, the total absorbance of the films as a function of molecular area suggests that a multilayer structure is formed between 40 and 46 mN m(-1).
引用
收藏
页码：627 / 630
页数：4
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