Potential of a white-rot fungus Pleurotus eryngii F032 for degradation and transformation of fluorene

被引：43

作者：

Hadibarata, Tony ^{[1
]}

Kristanti, Risky Ayu ^{[2
]}

机构：

[1] Univ Teknol Malaysia, Inst Environm & Water Resources Management, Utm Skudai 81310, Johor Bahru, Malaysia

[2] Osaka Univ, Div Sustainable Energy & Environm Engn, Suita, Osaka 5670871, Japan

来源：

FUNGAL BIOLOGY | 2014年 / 118卷 / 02期

关键词：

Fluorene biotransformation; 9-Fluorenone; Ligninolytic enzymes; Metabolic pathway; Benzoic acid; POLYCYCLIC AROMATIC-HYDROCARBONS; NAPHTHALENE METABOLISM; MYCOBACTERIUM SP; BIODEGRADATION; PHENANTHRENE; FLUORANTHENE; LIGNIN; PYRENE; IDENTIFICATION; PATHWAY;

D O I：

10.1016/j.funbio.2013.11.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The white-rot fungus Pleurotus eryngii F032 showed the capability to degrade a three fused-ring aromatic hydrocarbons fluorene. The elimination of fluorene through sorption was also investigated. Enzyme production is accompanied by an increase in biomass of P. eryngii F032 during degradation process. The fungus totally degraded fluorine within 23 d at 10-mg l(-1) solution. Fluorene degradation was affected with initial fluorene concentrations. The highest enzyme activity was shown by laccase in the 10-mg l(-1) culture after 30 d of incubation (1620 U l(-1)). Few activities of enzymes were observed in the fungal cell at the varying concentration of fluorene. Three metabolic were detected and separated in ethylacetate extract, after isolated by column chromatography. The metabolites, 9-fluorenone, phthalic acid, and benzoic acid were identified using UV-vis spectrophotometer and gas chromatography-mass spectrometry (GC MS). The results show the presence of a complex mechanism for the regulation of fluorene-degrading enzymes. (C) 2013 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：222 / 227

页数：6

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