EFFECTS OF MALEIMIDE-POLYETHYLENE GLYCOL-MODIFIED HUMAN HEMOGLOBIN (MP4) ON TISSUE NECROSIS IN SKH1-HR HAIRLESS MICE

被引：6

作者：

Goertz, O. ^{[1
]}

Kirschner, M. H. ^{[4
]}

Lilienfein, H. ^{[1
]}

Babilas, P. ^{[2
]}

Steinau, H. U. ^{[1
]}

Andree, C. ^{[5
]}

Daigeler, A. ^{[1
]}

Stachon, A. ^{[3
]}

Homann, H. ^{[1
]}

Langer, S. ^{[1
]}

机构：

[1] Ruhr Univ Bochum, Burn Ctr, Dept Plast & Hand Surg, Univ Hosp Bergmannsheil, D-4789 Bochum, Germany

[2] Univ Regensburg, Med Ctr, Dept Dermatol, D-8400 Regensburg, Germany

[3] Ruhr Univ Bochum, Inst Clin Chem Transfus & Lab Med, D-4789 Bochum, Germany

[4] BBD Aesculap GmbH, Tuttlingen, Germany

[5] Sana Hosp, Dusseldorf, Germany

来源：

EUROPEAN JOURNAL OF MEDICAL RESEARCH | 2009年 / 14卷 / 03期

关键词：

MP4; hairless mice; intravital fluorescent microscopy; oxygen carrier; free hemoglobin; intracardiac access; hemodilution; SKIN-FLAP; BLOOD-FLOW; MICROCIRCULATION; HEMODILUTION; REDUCTION; FAILURE; SAFETY; RATS;

D O I：

10.1186/2047-783X-14-3-123

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Objective: Tissue hypoxia after blood loss, replantation and flap reperfusion remains a challenging task in surgery. Normovolemic hemodilution improves hemorheologic properties without increasing oxygen carrying capacity. Red blood cell transfusion is the current standard of treatment with its attendant risks. The aim of this study was to investigate the potential of the chemically, modified hemoglobin, MP4, to reduce skin flap necrosis and its effect on selected blood markers and kidneys. Materials and Methods: Tissue ischemia was induced in the car of hairless mice (n=26). Hemodilution was performed by replacing one third of blood volume with the similar amount of MP4, dextran, or blood. The extent of non-perfused tissue was assessed by intravital fluorescent microscopy. Results: Of all groups, MP4 showed the smallest area of no perfusion (in percentage of the ear +/- SEM: 16.3% +/- 2.4), the control group the largest (22.4% +/- 3.5). Leukocytes showed a significant increase in the MP4 and dextran group (from 8.7 to 13.6 respectively 15.4*10(9)/1). On histology no changes of the kidneys could be observed. Conclusion: MP4 causes an increase of leukocytes, improves the oxygen supply of the tissue and shows no evidence of renal impairment.

引用

页码：123 / 129

页数：7

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