Metagenomic analysis of the human distal gut microbiome

被引：3412
作者：
Gill, Steven R.
Pop, Mihai
DeBoy, Robert T.
Eckburg, Paul B.
Turnbaugh, Peter J.
Samuel, Buck S.
Gordon, Jeffrey I.
Relman, David A.
Fraser-Liggett, Claire M.
Nelson, Karen E.
机构：
[1] Inst Genom Res, Rockville, MD 20850 USA
[2] Stanford Univ, Sch Med, Dept Med, Stanford, CA 94305 USA
[3] Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA
[4] Vet Affairs Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA
[5] Washington Univ, Sch Med, Ctr Genome Sci, St Louis, MO 63108 USA
[6] George Washington Univ, Sch Med, Dept Physiol & Pharmacol, Washington, DC 20037 USA
[7] George Washington Univ, Sch Med, Dept Microbiol & Trop Dis, Washington, DC 20037 USA
来源：
SCIENCE | 2006年 / 312卷 / 5778期
关键词：
D O I：
10.1126/science.1124234
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The human intestinal microbiota is composed of 10(13) to 10(14) microorganisms whose collective genome ("microbiome'') contains at least 100 times as many genes as our own genome. We analyzed similar to 78 million base pairs of unique DNA sequence and 2062 polymerase chain reaction amplified 16S ribosomal DNA sequences obtained from the fecal DNAs of two healthy adults. Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes. Our microbiome has significantly enriched metabolism of glycans, amino acids, and xenobiotics; methanogenesis; and 2-methyl-D-erythritol 4-phosphate pathway - mediated biosynthesis of vitamins and isoprenoids. Thus, humans are superorganisms whose metabolism represents an amalgamation of microbial and human attributes.
引用
收藏
页码：1355 / 1359
页数：5
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