A practical NMR-based high-throughput assay for screening enantioselective catalysts and biocatalysts

被引：2

作者：

Reetz, MT ^{[1
]}

Eipper, A ^{[1
]}

Tielmann, P ^{[1
]}

Mynott, R ^{[1
]}

机构：

[1] Max Planck Inst Kohlenforsch, D-45470 Mulheim, Germany

来源：

ADVANCED SYNTHESIS & CATALYSIS | 2002年 / 344卷 / 09期

关键词：

asymmetric catalysis; combinatorial catalysis; directed evolution; enzyme screening; NMR spectroscopy;

D O I：

10.1002/1615-4169(200210)344:9<1008::AID-ADSC1008>3.0.CO;2-T

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Two NMR-based approaches for high-throughput screening of enantioselective catalysts and biocatalysts are described. One version makes use of pseudo-enantiomers or pseudo-meso-compounds based on C-13-labeling. A throughput of at least 1400 ee determinations per day is possible by using an appropriate flow-through cell and an autosampler. The other approach is based on traditional diastereomer formation using a chiral reagent or complexing agent. The ee values are accurate to within +/-2% and +/-5% of the true values.

引用

页码：1008 / 1016

页数：9

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