Preventing the Solid Cancer Progression via Release of Anticancer-Cytokines in Co-Culture with Cold Plasma-Stimulated Macrophages

被引:61
作者
Kaushik, Nagendra Kumar [1 ]
Kaushik, Neha [2 ]
Adhikari, Manish [1 ]
Ghimire, Bhagirath [1 ]
Linh, Nguyen Nhat [1 ]
Mishra, Yogendra Kumar [3 ]
Lee, Su-Jae [2 ]
Ha Choi, Eun [1 ]
机构
[1] Kwangwoon Univ, Plasma Biosci Res Ctr, Appl Plasma Med Ctr, Dept Elect & Biol Phys, Seoul 01897, South Korea
[2] Hanyang Univ, Dept Life Sci, Lab Mol Biochem, Seoul 04763, South Korea
[3] Univ Kiel, Inst Mat Sci, Funct Nanomat, Kaiserstr 2, D-24143 Kiel, Germany
基金
新加坡国家研究基金会;
关键词
monocyte-macrophage stimulation; cancer inhibition; mesenchymal shift; cancer stem cells; DIFFERENTIATION; ACTIVATION; SHIFT;
D O I
10.3390/cancers11060842
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Non-thermal atmospheric pressure plasma sources operated in ambient environments are known to generate a variety of reactive oxygen and nitrogen species which could be applied for various biomedical applications. Herein, we fabricate a micro-dielectric barrier discharge plasma device by using screen-printing technology and apply it for studying immuno-stimulatory effects. We demonstrate a tumor-suppressive role for plasma-stimulated macrophages in metastatic solid cancers that directly elicit proliferation and are responsible for tumor relapse mediated by mesenchymal shift. Using microarray analysis, we observed that cold plasma stimulates and differentiates monocyte cells into macrophages as demonstrated by expression of several cytokine/chemokine markers. Moreover, plasma treatment stimulates the differentiation of pro-inflammatory (M1) macrophages to a greater extent. These stimulated macrophages favor anti-tumorigenic immune responses against metastasis acquisition and cancer stem cell maintenance in solid cancers in vitro. Differentiation of monocytes into anticancer macrophages could improve the efficacy of plasma treatment, especially in modifying pro-tumor inflammatory microenvironment through effecting highly resistant immunosuppressive tumor cells associated with tumor relapse.
引用
收藏
页数:22
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