Molecular characterization of Ciona sperm outer arm dynein reveals multiple components related to outer arm docking complex protein 2

被引:21
作者
Hozumi, Akiko
Satouh, Yuhkoh
Makino, Yumiko
Toda, Toshifusa
Ide, Hiroyuki
Ogawa, Kazuo
King, Stephen M.
Inaba, Kazuo
机构
[1] Univ Tsukuba, Shimoda Marine Res Ctr, Shizuoka 4150025, Japan
[2] Tohoku Univ, Dept Dev Biol & Neurosci, Grad Sch Life Sci, Sendai, Miyagi, Japan
[3] Natl Inst Basic Biol, Ctr Analyt Instruments, Okazaki, Aichi 444, Japan
[4] Tokyo Metropolitan Inst Gerontol, Proteom Collaborat Ctr, Tokyo, Japan
[5] Natl Inst Nat Sci, Ctr Radioisotope Facil, Okazaki, Aichi, Japan
[6] Univ Connecticut, Ctr Hlth, Farmington, CT USA
[7] Japan Sci & Technol Corp, CREST, Tokyo, Japan
来源
CELL MOTILITY AND THE CYTOSKELETON | 2006年 / 63卷 / 10期
关键词
sperm flagella; dynein; axoneme; Ciona intestinalis;
D O I
10.1002/cm.20146
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Using proteomic and immunochemical techniques, we have identified the light and intermediate chains (IC) of outer arm dynein from sperm axonemes of the ascidian Ciona intestinalis. Ciona outer arm dynein contains six light chains (LC) including a leucine-rich repeat protein, Tctex1- and Tctex2-related proteins, a protein similar to Drosophila roadblock and two components related to Chlamydomonas LC8. No LC with thioredoxin domains is included in Ciona outer ann dynein. Among the five ICs in Ciona, three are orthologs of those in sea urchin dynein: two are WD-repeat proteins and the third one, unique to metazoan sperm flagella, contains both thioredoxin and nucleoside diphosphate kinase modules. The remaining two Ciona ICs have extensive coiled coil structure and show sequence similarity to outer arm dynein docking complex protein 2 (DC2) that was first identified in Chlamydomonas flagella. We recently identified a third DC2-like protein with coiled coil structure, Ci-Axp66.0 that is also associated in substoichiometric amounts with Ciona outer arm dynein. In addition, Oda5p, a component of an additional complex required for assembly of outer ann dynein in Chlamyclomonas flagella, also groups with this family of DC2-like proteins. Thus, the assembly of outer arm dynein onto doublet microtubules involves multiple coiled-coil proteins related to DC2.
引用
收藏
页码:591 / 603
页数:13
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