Single-shot super-resolution total internal reflection fluorescence microscopy

被引：55

作者：

Guo, Min ^{[1
]}

Chandris, Panagiotis ^{[1
]}

Giannini, John Paul ^{[1
,2
]}

Trexler, Adam J. ^{[3
,8
]}

Fischer, Robert ^{[3
]}

Chen, Jiji ^{[4
]}

Vishwasrao, Harshad D. ^{[4
]}

Rey-Suarez, Ivan ^{[1
,2
]}

Wu, Yicong ^{[1
]}

Wu, Xufeng ^{[3
]}

Waterman, Clare M. ^{[3
]}

Patterson, George H. ^{[5
]}

Upadhyaya, Arpita ^{[6
,7
]}

Taraska, Justin W. ^{[3
]}

Shroff, Hari ^{[1
,4
,6
,7
]}

机构：

[1] Natl Inst Biomed Imaging & Bioengn, Sect High Resolut Opt Imaging, NIH, Bethesda, MD 20892 USA

[2] Univ Maryland, Biophys Program, College Pk, MD 20742 USA

[3] NHLBI, NIH, Bldg 10, Bethesda, MD 20892 USA

[4] NIH, Adv Imaging & Microscopy Resource, Bldg 10, Bethesda, MD 20892 USA

[5] Natl Inst Biomed Imaging & Bioengn, Sect Biophoton, NIH, Bethesda, MD USA

[6] Univ Maryland, Dept Phys, College Pk, MD 20742 USA

[7] Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA

[8] Northrop Grumman Corp, Monterey, CA USA

来源：

NATURE METHODS | 2018年 / 15卷 / 06期

基金：

美国国家科学基金会;

关键词：

STRUCTURED ILLUMINATION; PARTICLE TRACKING; GOLGI TRANSPORT; LIVE CELLS; PROTEINS; DISTRIBUTIONS; TRAFFICKING; MODULATOR; TIRF; RAS;

D O I：

10.1038/s41592-018-0004-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We combined instant structured illumination microscopy (iSIM) with total internal reflection fluorescence microscopy (TIRFM) in an approach referred to as instant TIRF-SIM, thereby improving the lateral spatial resolution of TIRFM to 115 +/- 13 nm without compromising speed, and enabling imaging frame rates up to 100 Hz over hundreds of time points. We applied instant TIRF-SIM to multiple live samples and achieved rapid, high-contrast super-resolution imaging close to the coverslip surface.

引用

页码：425 / +

页数：7

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