Kazrin, a novel periplakin-interacting protein associated with desmosomes and the keratinocyte plasma membrane

被引:51
作者
Groot, KR [1 ]
Sevilla, LM [1 ]
Nishi, K [1 ]
DiColandrea, T [1 ]
Watt, FM [1 ]
机构
[1] Canc Res UK, London Res Inst, Keratinocyre Lab, London WC2A 3PX, England
关键词
epidermis; cornified envelope; microvilli; actin; differentiation filament;
D O I
10.1083/jcb.200312123
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Periplakin forms part of the scaffold onto which the epidermal cornified envelope is assembled. The NH2-terminal 133 amino acids mediate association with the plasma membrane and bind a novel protein, kazrin. Kazrin is highly conserved and lacks homology to any known protein. There are four alternatively spliced transcripts, encoding three proteins with different NH2 termini. Kazrin is expressed in all layers of stratified squamous epithelia; it becomes membrane associated in the suprabasal layers, coincident with up-regulation of periplakin, and is incorporated into the cornified envelope of cultured keratinocytes. Kazrin colocalizes with periplakin and desmoplakin at desmosomes and with periplakin at the interdesmosomal plasma membrane, but its subcellular distribution is independent of periplakin. On transfection, all three kazrin isoforms have similar subcellular distributions. We conclude that kazrin is a novel component of desmosomes that associates with periplakin.
引用
收藏
页码:653 / 659
页数:7
相关论文
共 15 条
[1]   Periplakin gene targeting reveals a constituent of the cornified cell envelope dispensable for normal mouse development [J].
Aho, S ;
Li, KH ;
Ryoo, Y ;
McGee, C ;
Ishida-Yamamoto, A ;
Uitto, J ;
Klement, JF .
MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (14) :6410-6418
[2]  
AHO S, 2003, RECENT RES DEV CELL, V1, P133
[3]   ERM proteins and merlin: Integrators at the cell cortex [J].
Bretscher, A ;
Edwards, K ;
Fehon, RG .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (08) :586-599
[4]   Subcellular distribution of envoplakin and periplakin:: Insights into their role as precursors of the epidermal cornified envelope [J].
DiColandrea, T ;
Karashima, T ;
Määttä, A ;
Watt, FM .
JOURNAL OF CELL BIOLOGY, 2000, 151 (03) :573-585
[5]   Bridging cytoskeletal intersections [J].
Fuchs, E ;
Karakesisoglou, I .
GENES & DEVELOPMENT, 2001, 15 (01) :1-14
[6]   Intermediate filament-membrane attachments function synergistically with actin-dependent contacts to regulate intercellular adhesive strength [J].
Huen, AC ;
Park, JK ;
Godsel, LM ;
Chen, XJ ;
Bannon, LJ ;
Amargo, FV ;
Hudson, TY ;
Mongiu, AK ;
Leigh, IM ;
Kelsell, DP ;
Gumbiner, BM ;
Green, KJ .
JOURNAL OF CELL BIOLOGY, 2002, 159 (06) :1005-1017
[7]  
Kalinin A, 2001, J CELL SCI, V114, P3069
[8]   Epithelial barrier function: assembly and structural features of the cornified cell envelope [J].
Kalinin, AE ;
Kajava, AV ;
Steinert, PM .
BIOESSAYS, 2002, 24 (09) :789-800
[9]   Interaction of periplakin and envoplakin with intermediate filaments [J].
Karashima, T ;
Watt, FM .
JOURNAL OF CELL SCIENCE, 2002, 115 (24) :5027-5037
[10]   Plakins: a family of versatile cytolinker proteins [J].
Leung, CL ;
Green, KJ ;
Liem, RKH .
TRENDS IN CELL BIOLOGY, 2002, 12 (01) :37-45