PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity

被引：28

作者：

Chang, Ming ^{[1
,2
]}

Zhao, Jinping ^{[3
]}

Chen, Huan ^{[1
,2
]}

Li, Guangyong ^{[4
,5
]}

Chen, Jian ^{[1
,2
]}

Li, Min ^{[2
]}

Palmer, Ian A. ^{[2
]}

Song, Junqi ^{[3
,6
]}

Alfano, James R. ^{[4
,5
]}

Liu, Fengquan ^{[1
]}

Fu, Zheng Qing ^{[2
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Plant Protect, Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety,State Key Lab, Nanjing 210014, Jiangsu, Peoples R China

[2] Univ South Carolina, Dept Biol Sci, Columbia, SC 29208 USA

[3] Texas A&M Univ Syst, Texas A&M AgriLife Res Ctr Dallas, Dallas, TX 75252 USA

[4] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA

[5] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68588 USA

[6] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA

来源：

MOLECULAR PLANT | 2019年 / 12卷 / 05期

基金：

中国国家自然科学基金;

关键词：

PBS3; EDS1; NPR3; NPR4; the 26S proteasome; PAMP-triggered immunity; DISEASE RESISTANCE; SALICYLIC-ACID; DEFENSE RESPONSES; SIGNALING PATHWAYS; ARABIDOPSIS EDS1; CELL-DEATH; RECEPTORS; PERCEPTION; INFECTION; HORMONES;

D O I：

10.1016/j.molp.2019.01.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant immunity is controlled by both positive regulators such as PBS3 and EDS1 and negative regulators such as NPR3 and NPR4. However, the relationships among these important immune regulators remain elusive. In this study, we found that PBS3 interacts with EDS1 in both the cytoplasm and the nucleus, and is required for EDS1 protein accumulation. NPR3 and NPR4, which function as salicylic acid receptors and adaptors of Cullin3-based E3 ligase, interact with and mediate the degradation of EDS1 via the 26S proteasome. We further discovered that PBS3 inhibits the polyubiquitination and subsequent degradation of EDS1 by reducing the association of EDS1 with the Cullin3 adaptors NPR3 and NPR4. Furthermore, we showed that PBS3 and EDS1 also contribute to PAMP-triggered immunity in addition to effector-triggered immunity. Collectively, our study reveals a novel mechanism by which plants fine-tune defense responses by inhibiting the degradation of a positive player in plant immunity.

引用

页码：678 / 688

页数：11

共 46 条

[1] Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis [J].